Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WRKY33 Polyclonal antibody

Uniprot No.

Q8S8P5

Target Names

WRKY33

Alternative Names

WRKY33 antibody; At2g38470 antibody; T19C21.4 antibody; Probable WRKY transcription factor 33 antibody; WRKY DNA-binding protein 33 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana WRKY33 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Involved in defense responses. Required for resistance to the necrotrophic fungal pathogen B.cinerea. Regulates the antagonistic relationship between defense pathways mediating responses to the bacterial pathogen P. syringae and the necrotrophic pathogen B.cinerea. Required for the phytoalexin camalexin synthesis following infection with B.cinerea. Acts as positive regulator of the camalexin biosynthetic genes PAD3 (CYP71B15) and CYP71A13 by binding to their promoters. Acts downstream of MPK3 and MPK6 in reprogramming the expression of camalexin biosynthetic genes, which drives the metabolic flow to camalexin production. Functions with WRKY25 as positive regulator of salt stress response and abscisic acid (ABA) signaling. Functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway. The DNA-binding activity of WRKY33 is increased by SIB1 and SIB2.

Gene References into Functions

Binding of WRKY18, WRKY40, and WRKY33 to promoters of genes Implicated in microbe-associated molecular patterns (MAMPs)-triggered Immunity. PMID: 28011690
AtWRKY33 has an extended C-terminal domain (CTD) absent in its close homologue AtWRKY25. Both its CTD and the strong pathogen/stress-responsive expression of AtWRKY33 are necessary to complement the critical phenotypes of atwrky33. PMID: 25969555
Loss-of-WRKY33 function resulted in elevated abscisic acid levels and genetic studies confirmed that WRKY33 acts upstream of NCED3/NCED5 to negatively regulate abscisic acid biosynthesis. PMID: 26076231
The WRKY33 proteins interact with VQ motif of SIB1 (SIGMA FACTOR-INTERACTING PROTEIN1) proteins through their C-terminal WRKY domains. PMID: 22535423
direct binding of WRKY33 to the W-boxes in the promoters of ACS2 and ACS6 genes in vivo, suggesting that WRKY33 is directly involved in the activation of ACS2 and ACS6 expression downstream of MPK3/MPK6 cascade in response to pathogen invasion PMID: 22761583
WRKY33 is a negative regulator of the salicylic acid pathway upon Botrytis cinerea infection. PMID: 22392279
Dual-targeted SIB1 and SIB2 function as activators of WRKY33 in plant defense against necrotrophic pathogens. PMID: 21990940
WRKY33 is required for MPK3/MPK6-induced camalexin biosynthesis. PMID: 21498677
MKS1 function and subcellular location requires an intact N-terminus important for both MPK4 and WRKY33 interactions PMID: 21203436
Mmutations of WRKY33 gene encoding a WRKY transcription factor cause enhanced susceptibility to Botrytis cinerea and Alternaria brassicicola concomitant with reduced expression of the jasmonate-regulated plant defensin PDF1.2 gene. PMID: 17059405
Study shows that a set of three WRKY-specific cis-acting DNA elements (W boxes) within the AtWRKY33 promoter is required for efficient pathogen- or PAMP-triggered gene activation. PMID: 17427812

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Subcellular Location

Nucleus.

Protein Families

WRKY group I family

Tissue Specificity

Highly expressed in roots, leaves and flowers, and at lower levels in stems, siliques and seeds.

Database Links

KEGG: ath:AT2G38470

STRING: 3702.AT2G38470.1

UniGene: At.13123

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WRKY33 Antibody

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