WRKY53 Antibody

Datasheet

Code	CSB-PA890447XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) WRKY53 Polyclonal antibody

Uniprot No.

Q9SUP6

Target Names

WRKY53

Alternative Names

WRKY53 antibody; At4g23810 antibody; F9D16.280Probable WRKY transcription factor 53 antibody; WRKY DNA-binding protein 53 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana WRKY53 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. May regulate the early events of leaf senescence. Negatively regulates the expression of ESR/ESP. Together with WRKY46 and WRKY70, promotes resistance to P.syringae, probably by enhancing salicylic acid (SA)- dependent genes. Contributes to the suppression of jasmonic acid (MeJA)-induced expression of PDF1.2.

Gene References into Functions

The findings provide strong support for the direct regulatory roles of TGA3 and WRKY53 in the salicylic acid (SA) and NPR1-dependent activation of a Caulimoviral promoter (CmYLCV). PMID: 28913593
activated expression inhibits stomatal closure by reducing H2O2 content and facilitates stomatal opening by promoting starch degradation PMID: 25861729
WRKY53 is a master regulator of age-induced leaf senescence. PMID: 25395454
ATL31 plays a role in senescence together with the WRKY53 transcription factor. PMID: 24399238
WHY1 was an upstream regulator of WRKY53 during leaf senescence. PMID: 23922267
These results suggest that WRKY53 and ESR mediate negative crosstalk between pathogen resistance and senescence, which is most likely governed by the jasmonic acid and salicylic acid equilibrium. PMID: 17369373
WRKY53 and HSPRO2 appear to function downstream of salicylic acid and to be negatively regulated by signaling through jasmonic acid and ethylene. PMID: 17977154
Heterochromatic silencing by histone methylation of WRKY53 controls leaf senescence. PMID: 19143996

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Subcellular Location

Nucleus.

Protein Families

WRKY group III family

Database Links

KEGG: ath:AT4G23810

STRING: 3702.AT4G23810.1

UniGene: At.2572

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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WRKY53 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background