wtf10 Antibody

Code CSB-PA524897XA01SXV
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Product Details

Full Product Name Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) wtf10 Polyclonal antibody
Uniprot No. O74838
Target Names wtf10
Alternative Names wtf10; wtf7; SPCC1183.10; Meiotic drive suppressor wtf10
Raised in Rabbit
Species Reactivity Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) wtf10 protein
Immunogen Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Acts as a suppressor component of the dual wtf meiotic drive system, and can suppress but not confer meiotic drive by compatible poisons. Wtf meiotic drive systems promote unequal transmission of alleles from the parental zygote to progeny spores by encoding a poison and an antidote from the same locus; the poison is trans-acting and forms toxic aggregates in all spores within an ascus, wherease the antidote is spore-specific and targets aggregates for degradation by the vacuole. Meiotic drive by wtf systems therefore lead to poisoning of all progeny that do not inherit the dual poison/antidote allele, or express a compatible antidote.
Subcellular Location Spore membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein.
Protein Families WTF family
Database Links

KEGG: spo:SPCC1183.10

STRING: 4896.SPCC1183.10.1

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