mei-9 Antibody

Code CSB-PA634499XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) mei-9 Polyclonal antibody
Uniprot No.
Target Names
mei-9
Alternative Names
mei-9 antibody; CG3697 antibody; DNA repair endonuclease XPF antibody; EC 3.1.-.- antibody; Protein meiotic 9 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) mei-9 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Implicated in recombination events during meiosis, mostly in meiotic exchange. May directly resolve Holliday junctions within recombination intermediates leading to DNA exchange. Also required for the repair of mismatches within meiotic heteroduplex DNA and for nucleotide excision repair.
Gene References into Functions
  1. MUS312 facilitates resolution of meiotic Holliday junction intermediates by MEI-9 PMID: 12504024
  2. Drosophila mei-9 has roles in DNA repair and recombination, as shown by mutational analysis PMID: 15166153
  3. These results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic recombination, and, in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolution. PMID: 17339219
  4. HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers. PMID: 18957705
Subcellular Location
Nucleus.
Protein Families
XPF family
Database Links

KEGG: dme:Dmel_CG3697

STRING: 7227.FBpp0070624

UniGene: Dm.1684

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