xrn-2 Antibody

Code CSB-PA874711XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans xrn-2 Polyclonal antibody
Uniprot No.
Target Names
xrn-2
Alternative Names
xrn-2 antibody; Y48B6A.3 antibody; 5'-3' exoribonuclease 2 homolog antibody; EC 3.1.13.- antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans xrn-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Possesses 5'->3' exoribonuclease activity. Plays a role in maintenance of steady-state concentration and turnover of microRNAs (miRNA) by degradation of mature miRNA. Degradation role is enhanced when in complex with paxt-1. Partially redundant to xrn-1 in miRNA guide strand degradation. Implicated in differential regulation of mRNAs such as let-7 by controlling the accumulation of mature miRNA. Positively regulates molting of the pharyngeal cuticle.
Gene References into Functions
  1. In Caenorhabditis elegans, XRN-2 functions with PAXT-1, which shares a putative XRN-2-binding domain (XTBD) with otherwise unrelated mammalian proteins. The study characterizes the structure and function of an XTBD-XRN2 complex. PMID: 26779609
  2. XRN2 has unanticipated miRNA specificity in vivo, and its diverse developmental functions may relate to distinct substrates. PMID: 24445807
  3. degradation of mature miRNAs in the nematode Caenorhabditis elegans, mediated by the 5'-->3' exoribonuclease XRN-2, affects functional miRNA homeostasis in vivo PMID: 19734881
Subcellular Location
Nucleus.
Protein Families
5'-3' exonuclease family, XRN2/RAT1 subfamily
Tissue Specificity
Expressed in the pharyngeal myoepithelium and intestine. Also expressed in several anterior neurons including the sensory neurons, as well as the interneuron PVT and the pharyngeal motorneuron M5.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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