xylE Antibody

1. Molecular dynamics (MD) study of an all-atom model system to elucidate the atomistic details and the free-energy landscape along the path of binding a xylopyranose from the extracellular space to the inside of the transporter protein XylE. From the MD simulations, the Gibbs free energy of binding was found to be -4.4kcal/mol in agreement with the experimental value of -4.7kcal/mol. PMID: 29032199
2. The models and subsequent experimental validation suggest that multiple residue substitutions are required to produce the thermodynamic and functional distinction between XylE and GLUT1. Despite the lack of simulation studies with substrates, these computational and biochemical characterizations provide unprecedented insight into the mechanistic difference between proton symporters and uniporters. PMID: 28617850
3. Studied conformational states and homology of LacY and XylE. PMID: 25586173
4. The movement of XylE is described that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. PMID: 25088546
5. This current model helps to unify the apparently opposing concepts of alternate access and multisite models of ligand transport. PMID: 25163893
6. Authors present two structures of the major facilitator (MFS) xylose transporter XylE from Escherichia coli in inward open and partially occluded inward open conformations. PMID: 23624861

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KEGG: ecj:JW3991

STRING: 316385.ECDH10B_4220