BSK1 Antibody

Code CSB-PA850061XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BSK1 Polyclonal antibody
Uniprot No.
Target Names
BSK1
Alternative Names
BSK1 antibody; At4g35230 antibody; F23E12.210Serine/threonine-protein kinase BSK1 antibody; EC 2.7.11.1 antibody; Brassinosteroid-signaling kinase 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BSK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Serine/threonine kinase that acts as positive regulator of brassinosteroid (BR) signaling downstream of the receptor kinase BRI1. Mediates signal transduction from BRI1 by functioning as substrate of BRI1. Functions as a positive regulator of plant immunity. May be involved in the regulation of pattern-triggered immunity (PTI) downstream of the flagellin receptor FLS2. Possesses kinase activity in vitro. Kinase activity is required for its function in innate immunity.
Gene References into Functions
  1. The ability of the kinase domain of BRI1 to generate cGMP depends on its kinase catalytic ability. Furthermore, cGMP stimulates phosphorylation of BSK1. Therefore, cGMP is proposed to act as a modulator to stimulate activation of downstream phosphorylation of members of the BRI1 signaling pathway. PMID: 28482142
  2. Disruption of BSK1 membrane localization affects the BSK1-FLS2 interactions, suggesting the membrane association of BSK1 is important for both edr2-mediated signaling. PMID: 23733062
  3. BSK1 is a positive regulator of defense responses and has a significant role in pathogen-associated molecular pattern-trigged immunity. PMID: 23532072
  4. is involved in brassinosteroid signal transduction PMID: 20616146
  5. 3 homologous brassinosteroid (BR)-signaling kinases(BSK1 encoded by At4g35230, BSK2, BSK3)were identified; the BSKs are phosphorylated by BRI1; results demonstrate that BSKs are the substrates of BRI1 kinase that activate downstream BR signal transduction PMID: 18653891

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Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Database Links

KEGG: ath:AT4G35230

STRING: 3702.AT4G35230.1

UniGene: At.19802

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