YAK1 Antibody

Code CSB-PA320409XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YAK1 Polyclonal antibody
Uniprot No.
Target Names
YAK1
Alternative Names
YAK1 antibody; YJL141C antibody; J0652 antibody; Dual specificity protein kinase YAK1 antibody; EC 2.7.12.1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YAK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negative regulator of the cell cycle acting downstream of the cAMP-dependent protein kinase. Part of a glucose-sensing system involved in growth control in response to glucose availability. Phosphorylates POP2.
Gene References into Functions
  1. Yak1 is at the center of a regulatory cascade for adhesive growth and stress resistance PMID: 21149646
  2. Data discovered that six non-essential protein kinase (PKase) disruptions (Deltabck1, Deltamkk1, Deltaslt2/Deltampk1, Deltamck1, Deltassk2 and Deltayak1) suppressed the Cas-phenotype of the Deltaptp2 Deltamsg5 double disruptant. PMID: 20054684
  3. Yak1 kinase phosphorylates and activates Hsf1 under the conditions of low PKA activity. It can also activate Msn2, possibly by direct phosphorylation. PMID: 18793336
Subcellular Location
Cytoplasm. Nucleus. Note=Shuttles between both compartments in response to glucose.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily
Database Links

KEGG: sce:YJL141C

STRING: 4932.YJL141C

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