YAP5 Antibody

Code CSB-PA340276XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YAP5 Polyclonal antibody
Uniprot No.
Target Names
YAP5
Alternative Names
YAP5 antibody; YIR018W antibody; AP-1-like transcription factor YAP5 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YAP5 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator involved in the regulation of genes expressed in response to environmental changes and metabolic requirements. According to genome-wide promoter binding and gene expression studies it is a coregulator for the expression of ribosomal genes, while its own expression is induced by the cell cycle specific activator SBF (SWI4-SWI6).
Gene References into Functions
  1. Fe/S cluster binding to Yap5-activator domain induces a conformational change that may activate transcription PMID: 25368382
  2. loss of activity of the cytosolic iron-sulfur cluster assembly complex proteins or deletion of cytosolic glutaredoxins did not reduce expression of Yap5 target genes PMID: 22915593
  3. we propose that under Fe-adequacy Yap5 is activated leading to the upregulation of CCC1 with the consequent accumulation of Fe in the vacuole and deprivation of the cytosolic iron pool. PMID: 22616008
  4. the Yap5-mediated induction of TYW1 provides protection from high iron toxicity by the consumption of free cytosolic iron through the formation of protein-bound iron-sulfur clusters PMID: 21917924
  5. These results show that Yap5 is an iron-sensing transcription factor and that iron regulates transcriptional activation. PMID: 18070921

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
BZIP family, YAP subfamily
Database Links

KEGG: sce:YIR018W

STRING: 4932.YIR018W

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