ybaK Antibody

Code CSB-PA364540XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) ybaK Polyclonal antibody
Uniprot No.
Target Names
ybaK
Alternative Names
ybaK antibody; b0481 antibody; JW0470 antibody; Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK antibody; EC 4.2.-.- antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) ybaK protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions in trans to edit the amino acid from incorrectly charged Cys-tRNA(Pro) via a Cys-tRNA(Pro) deacylase activity. May compensate for the lack of Cys-tRNA(Pro) editing by ProRS. Is also able to deacylate Cys-tRNA(Cys), and displays weak deacylase activity in vitro against Gly-tRNA(Gly), as well as, at higher concentrations, some other correctly charged tRNAs. Unlike some of its orthologs it is not able to remove the amino acid moiety from incorrectly charged Ala-tRNA(Pro).
Gene References into Functions
  1. data suggest that YbaK-mediated hydrolysis of aminoacyl-tRNA can influence cell growth PMID: 15886196
Subcellular Location
Cytoplasm.
Protein Families
Prolyl-tRNA editing family, YbaK/EbsC subfamily
Database Links

KEGG: ecj:JW0470

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