y Antibody

Code CSB-PA362843XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) y Polyclonal antibody
Uniprot No.
Target Names
y
Alternative Names
y antibody; CG3757 antibody; Protein yellow antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) y protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Controls the pigmentation pattern of the adult cuticle and larval mouth parts.
Gene References into Functions
  1. First, the authors show that y is required for the production of black Dopamine-melanin. Then, using in situ hybridization, they show that the expression of y is strongly modulated by temperature in the abdominal epidermis of pharate females but not in bristles. PMID: 28230190
  2. the negative effect of transcription of the Drosophila genes white and yellow remained, but weakened with increased distance between the leading promoter and enhancer and with decreased distance between the enhancer and target promoter PMID: 27183791
  3. role of yellow gene sequences from -70 to-146 bp in the transcriptional activation at the end of terminally truncated chromosome in Drosophila melanogaster PMID: 26163206
  4. Study shows that the polyadenylation signal from the yellow gene cannot efficiently terminate transcription when located in the gene intron and that the Su(Hw)-dependent 1A2 insulator cannot enhance the polyadenylation signal efficiency PMID: 24993965
  5. Data show that flies lacking yar RNAs are viable and show no overt morphological defects, consistent with maintained transcriptional regulation of the adjacent yellow (y) and achaete (ac) genes. PMID: 21775470
  6. Both insulators support basal activity of the yellow and white promoters in eyes. PMID: 21862564
  7. Data indicate that homolog pairing at yellow is efficient, as complementation was disrupted only in the presence of chromosomal rearrangements that break PMID: 19667134
  8. yellow transvection can occur at multiple genomic locations and indicate that the Drosophila genome generally is permissive to enhancer action in trans PMID: 11904429
  9. Drosophila y(yellow) and e(ebony) genes play critical, reciprocal roles in the formation of melanin patterns. PMID: 11934851
  10. Immunohistochemical colocalization of Yellow and male-specific Fruitless in Drosophila melanogaster neuroblasts PMID: 12054512
  11. Evolutionary changes in Yellow expression correlate with divergent melanin patterns and are a result of evolution in both cis- and trans-regulation. PMID: 12372246
  12. the data of this study suggest that y is a downstream member of the fru branch of the D. melanogaster sex determination hierarchy, where it plays a currently unknown role in the development of adult male wing extension during courtship. PMID: 12605459
  13. how the position in the genome affects the extent of repression of the yellow gene by the Su(Hw) insulator PMID: 12856417
  14. proposed that Yellow acts as a growth factor- or hormone-like molecule in the development of pigmentation and sexual behavior; discussed the consistency of this theory with experimental observations in flies and humans PMID: 14575686
  15. Multiple regulatory elements in the promoter region of the yellow gene. PMID: 15714950
  16. Contains a regulatory sequence that is required for normal male mating success. PMID: 16272418
  17. The effects of genotype of the strains, C(1)DX, ywf/Y, 23.5 MRF/CyL4, and C(1)DX,yf; pi2, on locus-specific instability in the yellow gene of the strains y(2-717, y(2-715), and y(2-700 ) from Uman' population of Drosophila melanogaster was studied. PMID: 17899812
  18. An increased amount of AUBERGINE interferes with the regular functioning of the somatic RNA interference pathway. PMID: 18385112
  19. Yellow locus residing at the very end of X chromosome (region 1A5-8 of cytologic map) carries significantly more transposon than retrotransposon-induced mutations, as compared to white locus (regions 3C2). PMID: 18822793

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Subcellular Location
Secreted.
Protein Families
Major royal jelly protein family
Database Links

KEGG: dme:Dmel_CG3757

STRING: 7227.FBpp0070070

UniGene: Dm.23761

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