yhaV Antibody

Datasheet

Code	CSB-PA353743XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) yhaV Polyclonal antibody

Uniprot No.

P64594

Target Names

yhaV

Alternative Names

yhaV antibody; b3130 antibody; JW3099 antibody; Ribonuclease toxin YhaV antibody; EC 3.1.-.- antibody; Ribonuclease YhaV antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) yhaV protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Toxic component of a type II toxin-antitoxin (TA) system. Has RNase activity in vitro. Overexpression leads to growth arrest after 30 minutes; these effects are overcome by concomitant expression of antitoxin SohA (PrlF). Massive overexpression is toxic. Unlike most other characterized TA systems degrades rRNA, and co-folding of the both TA proteins is necessary in vitro for inhibition of the RNase activity. It is not known if it has any sequence-specificity. Acts as a transcription factor. The YhaV/PrlF complex binds the prlF-yhaV operon, probably negatively regulating its expression.

Gene References into Functions

These results suggest that the YhaV C-terminal region is essential for mRNA interferase activity, and the W143 or H154 residues may play an analogous role to Y87 of RelE. PMID: 29642286
Data show that prlF and yhaV encode a novel toxin-antitoxin system in Escherichia coli. PMID: 17706670

Database Links

KEGG: ecj:JW3099

STRING: 316385.ECDH10B_3303

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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yhaV Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background