KCH1 Antibody

Code CSB-PA341668XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KCH1 Polyclonal antibody
Uniprot No.
Target Names
KCH1
Alternative Names
KCH1; YJR054W; J1669; Low affinity K(+ transporter 1
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KCH1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Low affinity potassium transporter that, with PRM6/KCH2, participates in high-affinity Ca(2+) influx system (HACS) activation during the response to mating pheromone. Directly promotes K(+) influx and HACS may electrochemically respond to this K(+) influx. KCH1 and KCH2 act at the apex of the calcium signaling pathway that is used for survival during prolonged exposures to mating pheromones.
Gene References into Functions
  1. These data demonstrate that the Kch1 and Kch2 proteins significantly contribute to the maintenance of optimal cation homeostasis and membrane potential in Saccharomyces cerevisiae but not in Candida albicans. PMID: 28810704
  2. Kch1 family proteins represent a conserved linkage between HACS and only certain classes of ER stress in these yeasts. PMID: 24142703
  3. Kch1 and Kch2 directly promote potassium influx and calcium signaling may electrochemically respond to potassium influx. PMID: 23204190
Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Database Links

KEGG: sce:YJR054W

STRING: 4932.YJR054W

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