Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) yjjG Polyclonal antibody

Uniprot No.

P0A8Y1

Target Names

yjjG

Alternative Names

yjjG antibody; b4374 antibody; JW4336 antibody; Pyrimidine 5'-nucleotidase YjjG antibody; EC 3.1.3.5 antibody; House-cleaning nucleotidase antibody; Non-canonical pyrimidine nucleotide phosphatase antibody; Nucleoside 5'-monophosphate phosphohydrolase antibody; dUMP phosphatase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) yjjG protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Nucleotidase that shows high phosphatase activity toward non-canonical pyrimidine nucleotides and three canonical nucleoside 5'-monophosphates (UMP, dUMP, and dTMP), and very low activity against TDP, IMP, UDP, GMP, dGMP, AMP, dAMP, and 6-phosphogluconate. Appears to function as a house-cleaning nucleotidase in vivo, since the general nucleotidase activity of YjjG allows it to protect cells against non-canonical pyrimidine derivatives such as 5-fluoro-2'-deoxyuridine, 5-fluorouridine, 5-fluoroorotate, 5-fluorouracil, and 5-aza-2'-deoxycytidine, and prevents the incorporation of potentially mutagenic nucleotides into DNA. Its dUMP phosphatase activity that catalyzes the hydrolysis of dUMP to deoxyuridine is necessary for thymine utilization via the thymine salvage pathway. Is strictly specific to substrates with 5'-phosphates and shows no activity against nucleoside 2'- or 3'-monophosphates.

Gene References into Functions

Blockage of the yjjG pathway for deoxyribose-1-phosphate generation greatly exacerbated the severity of thymine starvation in enriched media, and under these conditions the dgt pathway becomes crucial in protecting the cells against thymineless death. PMID: 21736641
YjjG, a dUMP phosphatase, is critical for thymine utilization by Escherichia coli K-12. PMID: 17189366
The substrate spectrum of YjjG suggests that its in vivo substrates are noncanonical pyrimidine derivatives, which might also include oxidized nucleobases such as 5-formyluracil and 5-hydroxyuracil. PMID: 17286574

Subcellular Location

Cytoplasm.

Protein Families

HAD-like hydrolase superfamily, YjjG family

Database Links

KEGG: ecj:JW4336

STRING: 316385.ECDH10B_4531

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yjjG Antibody

Product Details

Customer Reviews and Q&A

Target Background