LMO1 Antibody

Code CSB-PA963426XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LMO1 Polyclonal antibody
Uniprot No.
Target Names
LMO1
Alternative Names
LMO1 antibody; YLL007C antibody; L1353 antibody; ELMO family protein LMO1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LMO1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Forms a transiant heterodimeric complex with DCK1, that acts as a guanine nucleotide exchange factor (GEF) for the small GTPase RHO5. DCK1, LMO1 and RHO5 relocate to mitochondria upon oxidative stress and trigger cell death. The DCK1/LMO1/RHO5 signaling module mediates mitochondrial turnover under nitrogen starvation conditions via mitophagy. The DCK1/LMO1/RHO5 signaling module plays also a function in cell wall integrity signaling.
Gene References into Functions
  1. Rho5 rapidly relocates from the plasma membrane to mitochondria upon glucose starvation, mediated by its dimeric GDP/GTP exchange factor (GEF) Dck1/Lmo1. PMID: 30049968
  2. A regulatory role in autophagy/mitophagy is suggested by the colocalization of Rho5 with autophagic markers and the decreased mitochondrial turnover observed in dck1, lmo1 and rho5 deletion mutants. Rho5 activation may thus serve as a central hub for the integration of different signaling pathways. PMID: 25598154
Subcellular Location
Cytoplasm. Mitochondrion.
Database Links

KEGG: sce:YLL007C

STRING: 4932.YLL007C

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