EUC1 Antibody

Code CSB-PA311995XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EUC1 Polyclonal antibody
Uniprot No.
Target Names
EUC1
Alternative Names
EUC1 antibody; YMR111C antibody; YM9718.10CTranscription factor-like protein EUC1 antibody; Enriches ubiquitin on chromatin protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EUC1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor-like protein that binds to specific DNA motifs called ub-HS-motif associated with several locations where proteins other than histone H2B are ubiquitinated (ub-hotspots). Ubiquitination at these sites depends on the SUMO-targeted ubiquitin ligase (STUbL) complex SLX5/SLX8 and protein turnover on the CDC48 segregase. UBC9, SIZ1, or SIZ2 sumoylate DNA-bound EUC1 to stabilize its DNA-binding. Sumoylated EUC1 acts a cofactor required for the recruitment of the SLX5/SLX8 STUbL complex via specific contacts between EUC1 and SLX5, as well as an additional SUMO-mediated interaction. SLX5/SLX8 then ubiquitinates EUC1 and presumably other targets at ub-hotspots, and the CDC48/UFD1/NPL4 complex, together with UBX4 and UBX5, removes Lys-48-linked ubiquitinated proteins from chromatin. Ubiquitinated proteins could be either degraded by the proteasome or recycled by deubiquitination. EUC1 itself does not seem to underlie extensive turnover, as it is a very stable protein. EUC1 is able to act as a transcription factor, but its function at ub-hotspots does not seem to depend on this ability. EUC1-mediated ub-hotspots are crucial during stress responses when gene expression control is impaired.
Subcellular Location
Chromosome.
Database Links

KEGG: sce:YMR111C

STRING: 4932.YMR111C

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