YPC1 Antibody

Code CSB-PA334403XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPC1 Polyclonal antibody
Uniprot No.
Target Names
YPC1
Alternative Names
YPC1; YBR183W; YBR1305; Alkaline ceramidase YPC1; Acyl-CoA-independent ceramide synthase
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPC1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Alkaline ceramidase that hydrolyzes phytoceramide and also dihydroceramide into phytosphingosine or dihydrosphingosine. Prefers phytoceramide. Has also reverse activity as acyl-CoA-independent ceramide synthase, catalyzing synthesis of phytoceramide and dihydroceramide from palmitic acid and phytosphingosine or dihydrosphingosine. Is not responsible for the breakdown of unsaturated ceramide. Preferentially uses very long chain fatty acids (C-24 and C-26) in vivo compared to C-16 in vitro.
Gene References into Functions
  1. Ceramides of ypc1ydc1 remained normal even in presence of aureobasidin A, an inhibitor of inositolphosphorylceramide synthase. PMID: 24866405
  2. Sphingolipid homeostasis is studied by determination of membrane topology of the conserved residues of Ypc1. PMID: 23445175
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Alkaline ceramidase family
Database Links

KEGG: sce:YBR183W

STRING: 4932.YBR183W

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