YPD1 Antibody

Code CSB-PA293681XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPD1 Polyclonal antibody
Uniprot No.
Target Names
YPD1
Alternative Names
YPD1 antibody; YDL235C antibody; D0790 antibody; Phosphorelay intermediate protein YPD1 antibody; Histidine-containing phosphotransfer protein YPD1 antibody; Tyrosine phosphatase-dependent protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPD1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Phosphorelay intermediate protein that is part of the branched SLN1-YPD1-SKN7/SSK1 two-component regulatory system, which controls activity of the HOG1 pathway and gene expression in response to changes in the osmolarity of the extracellular environment. Catalyzes the phosphoryl group transfer from the membrane-bound osmosensing histidine kinase SLN1 to two distinct response regulator proteins, SSK1 in the cytoplasm, and transcription factor SKN7 in the nucleus.
Gene References into Functions
  1. Kinetic analysis of a eukaryotic "two-component" histidine-aspartic acid phosphotransfer system, enabling a comparison of the transfer rates and binding constants to the few bacterial systems that have been studied this way. PMID: 15628880
  2. analysis of the common docking site for response regulators on the yeast phosphorelay protein YPD1 PMID: 15769590
  3. Crystal structure of a complex between the phosphorelay protein YPD1 and the response regulator domain of SLN1 bound to a phosphoryl analog is reported. PMID: 18076904
Subcellular Location
Cytoplasm. Nucleus. Note=Localizes constitutively to the cytoplasm and the nucleus, independent on osmotic conditions and phosphorylation status of the protein.
Protein Families
YPD1 family
Database Links

KEGG: sce:YDL235C

STRING: 4932.YDL235C

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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