YPT11 Antibody

Code CSB-PA342566XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPT11 Polyclonal antibody
Uniprot No.
Target Names
YPT11
Alternative Names
YPT11 antibody; YNL304W antibody; N0410 antibody; GTP-binding protein YPT11 antibody; Rab GTPase YPT11 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YPT11 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in the positive control of both endoplasmic reticulum (ER) and mitochondrion inheritance during cell divison. Required for the MYO2-dependent retention of newly inherited mitochondria at the bud tip in developing daughter cells.
Gene References into Functions
  1. The abundance of active Ypt11 forms is controlled by phosphorylation status and degradation. PMID: 23427260
  2. Myo2 drives anterograde mitochondrial movements in budding yeast and this activity is supported by Ypt11 and Mmr1. PMID: 21807878
  3. we show that, in human cells, after heterologous expression of GFP-Ypt11 (where GFP stands for green fluorescent protein), the protein is targeted to transferrin-positive recycling endosomes. PMID: 15850457
  4. required for the vectorial actin-dependent movement of the late Golgi from the mother cell toward the emerging bud PMID: 18595704
Subcellular Location
Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Bud tip. Bud neck. Note=Enriched in the peripheral ER of small buds and daughter cells. Concentrates at the site of bud emergence, at the bud tip of the growing bud, and at the bud neck during M phase. Interaction with MYO2 is required for proper localization to the bud.
Protein Families
Small GTPase superfamily, Rab family
Database Links

KEGG: sce:YNL304W

STRING: 4932.YNL304W

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