YUC1 Antibody

Code CSB-PA886832XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) YUC1 Polyclonal antibody
Uniprot No.
Target Names
YUC1
Alternative Names
YUC1 antibody; YUC antibody; YUCCA antibody; YUCCA1 antibody; At4g32540 antibody; L23H3.20 antibody; Probable indole-3-pyruvate monooxygenase YUCCA1 antibody; EC 1.14.13.168 antibody; Flavin-containing monooxygenase YUCCA1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana YUC1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in auxin biosynthesis, but not in the tryptamine or the CYP79B2/B3 branches. Catalyzes in vitro the N-oxidation of tryptamine to form N-hydroxyl tryptamine. Involved during embryogenesis and seedling development. Required for the formation of floral organs and vascular tissues. Belongs to the set of redundant YUCCA genes probably responsible for auxin biosynthesis in shoots.
Gene References into Functions
  1. Data show that endoplasmic reticulum (ER) membrane anchored YUC proteins can mainly be found in roots, while cytosolic proteins are more abundant in the shoot. PMID: 28820425
  2. Studies indicate that YUCCA, a flavin-containing monooxygenase (YUC), catalyzes the last step of conversion from indole-3-pyruvate (IPyA) to indole-3-acetic acid (IAA). PMID: 26402640
  3. The induction of the YUCCA (YUC) genes increases Phenylacetic acid (PAA) metabolite levels in Arabidopsis, indicating that YUC flavin-containing monooxygenases may play a role in PAA biosynthesis. PMID: 26076971
  4. Yucasin is a potent inhibitor of YUC enzymes that offers an effective tool for analyzing the contribution of Indole-3-acetic acid (IAA) biosynthesis via the indole-3-pyruvic acid (IPyA) pathway to plant development and physiological processes. PMID: 24299123
  5. The analysis indicated that YUCCAs and TAA1, working in the IPA-YUC auxin biosynthesis pathway, are associated with SE induction, and that the expression of three YUCCA genes (YUC1, YUC4 and YUC10) is associated with LEC2 activity. PMID: 23722561
  6. YUCCAs (YUCs) encoding key enzymes in auxin biosynthesis are required for somatic embryo induction PMID: 23271028
  7. Data indicate that mature YUCCA 1 (YUC1) auxin biosynthesis gene overexpressing plants have elevated auxin levels and enhanced susceptibility to Pseudomonas syringae strain DC3000. PMID: 23521356
  8. YUCCA1 flavin monooxygenase functions in the indole-3-pyruvic acid branch of auxin biosynthesis PMID: 22108406
  9. Residues near the putative GXGXXG FAD binding site and the putative GXGXXG NADPH binding site are highly conserved in YUC flavin monooxygenases. PMID: 21205174
  10. These data demonstrate that auxin synthesized by the YUC flavin monooxygenases is an essential auxin source for Arabidopsis thaliana embryogenesis and postembryonic organ formation. PMID: 17704214

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Protein Families
FMO family
Tissue Specificity
Expressed in the apical meristems and young floral primordia. Detected in the floral meristems and at the base of the floral organs.
Database Links

KEGG: ath:AT4G32540

STRING: 3702.AT4G32540.1

UniGene: At.54581

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