Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ZUO1 Polyclonal antibody

Uniprot No.

P32527

Target Names

ZUO1

Alternative Names

ZUO1 antibody; YGR285CZuotin antibody; DnaJ-related protein ZUO1 antibody; J protein ZUO1 antibody; Heat shock protein 40 homolog ZUO1 antibody; Ribosome-associated complex subunit ZUO1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ZUO1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the ribosome-associated complex (RAC), a heterodimeric chaperone complex involved in regulation of accurate translation termination and in folding or maintaining nascent polypeptides in a folding-competent state. RAC stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones SSB1/SSB2 that bind to the nascent polypeptide chain. ZUO1 can act as a J-protein for SSB1/SSB2 only when associated with SSZ1.

Gene References into Functions

Ssz1 is catalytically inert and cannot adopt the closed conformation, but the substrate binding domain beta is completed by Zuo1. PMID: 28067917
This study presents new structural and cross-linking data allowing more precise positioning of Saccharomyces cerevisiae ribosome-associated J protein-Hsp70 chaperone Zuo1 near the 60S polypeptide-exit site and suggesting interactions of Zuo1 with the ribosomal protein eL31 and 25S rRNA helix 24. PMID: 27669034
The yeast ribosome-associated complex is composed of HSP40 (Zuotin) and HSP70 (Ssz1). RAC stabilizes the 80S ribosome in nonrotated, classical conformation. PMID: 25362488
study presents structural analyses of the ribosome-associated complex (RAC) consisting (Hsp70) Ssz1 and the Hsp40 Zuo1; a unique alpha-helical domain in Zuo1 mediates ribosome interaction of RAC near the ribosomal proteins L22e and L31e and ribosomal RNA helix H59 PMID: 23202586
Zuo1 activates Pdr1 causing premature growth arrest of cells during the diauxic shift, through quorum sensing, as they adapt to the changing environmental conditions. PMID: 22203981
Both Jjj1 and Zuo1 associate with nuclear 60S ribosomal biogenesis intermediates and play an important role in nuclear rRNA processing, leading to mature 25S rRNA PMID: 20368619
structural analysis of teh ribosome-associated complex (RAC) reveals an unusual Hsp70/Hsp40 interaction between zuotin and ssz PMID: 19920147
Zuo1 efficiently stimulates the ATPase activity of Ssb only when in complex with another Hsp70, Ssz1. PMID: 15908962
A plausible role of the ZUO1 chaperone is to stabilize genetic networks, thus making them more tolerant to malfunctioning of their constituents. PMID: 16849597

Hide All

Subcellular Location

Cytoplasm.

Database Links

KEGG: sce:YGR285C

STRING: 4932.YGR285C

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ZUO1 Antibody

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