aroA Antibody

Code CSB-PA363843ZA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) aroA Polyclonal antibody
Uniprot No.
Target Names
aroA
Alternative Names
3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS), aroA
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli aroA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
Gene References into Functions
  1. A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants. PMID: 22715408
  2. The results suggest that there is a direct relationship between stability of 5-enolpyruvylshikimate 3-phosphate synthase and pH stability of its folding intermediates PMID: 21327882
  3. We identified a novel RPMXR motif that is strictly conserved among class II EPSP synthases and is required for enzymatic acitvity and glyphosate resistance. PMID: 19799945
  4. glyphosate ternary complexes of P101S and P101L 5-enolpyruvylshikimate-3-phosphate synthase were determined to between 1.5- and 1.6-A resolution PMID: 17855366
  5. analysis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli PMID: 19211556
  6. In an active site that is overwhelmingly positively charged, Asp-313 and Glu-341 side chains of aroA form an "electrostatic sandwich" around the positive charge at C2 in cationic intermediates/transition states, stabilizing them and promoting catalysis. PMID: 19271774

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Subcellular Location
Cytoplasm.
Protein Families
EPSP synthase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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