iolO Antibody

Code CSB-PA893461ZA01TNJ
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Product Details

Full Product Name
Rabbit anti-Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) iolO Polyclonal antibody
Uniprot No.
Target Names
iolO
Alternative Names
5-keto-L-gluconate epimerase (EC 5.1.3.-) (Bifunctional nonphosphorylated sugar isomerase) (D-erythrose/D-threose isomerase) (L-ribulose 3-epimerase) (R3E) (Nonphosphorylated sugar 3-epimerase) (EC 5.1.3.-) (Nonphosphorylated sugar aldose-ketose isomerase) (EC 5.3.1.-), iolO
Raised in
Rabbit
Species Reactivity
Thermotoga maritima
Immunogen
Recombinant Thermotoga maritima iolO protein
Immunogen Species
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Catalyzes the reversible epimerization between 5-keto-L-gluconate (5-dehydro-L-gluconate) and D-tagaturonate, and thus probably functions in a myo-inositol degradation pathway together with IolG, IolM and IolN. Is not active on the enantiomer 5-keto-D-gluconate. Was also shown to be a nonphosphorylated sugar isomerase with broad substrate specificity in vitro. Is able to catalyze the reversible C3-epimerization of L-ribulose to L-xylulose, D-ribulose to D-xylulose, D-psicose to D-fructose, and D-tagatose to D-sorbose, with a substrate preference for ketopentoses rather than ketohexoses. Also catalyzes the aldose-ketose isomerization reaction from either D-erythrose or D-threose to D-erythrulose. Exhibits no activity for C4-epimerization of D-tagatose to D-fructose.
Gene References into Functions
  1. TM0416 is a putative nonphosphorylated sugar epimerase and catalyzes various C5 and C6 epimerization reactions. PMID: 28258150
Protein Families
Hyi family
Database Links

KEGG: tma:TM0416

STRING: 243274.TM0416

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