oprP Antibody

Code CSB-PA361596ZA01EZX
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Product Details

Full Product Name
Rabbit anti-Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) oprP Polyclonal antibody
Uniprot No.
Target Names
oprP
Alternative Names
oprP antibody; PA3279 antibody; Porin P antibody; Outer membrane protein D1 antibody
Raised in
Rabbit
Species Reactivity
Pseudomonas aeruginosa
Immunogen
Recombinant Pseudomonas aeruginosa oprP protein
Immunogen Species
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Anion specific, the binding site has higher affinity for phosphate than chloride ions. Porin O has a higher affinity for polyphosphates (tripolyphosphate and pyrophosphate) while porin P has a higher affinity for orthophosphate.
Gene References into Functions
  1. Our analyzes show that OprO, and to a lesser degree OprP, have unexpected very high permeability to fosfomycin, so the antibiotic should be a potentially excellent alternative choice for the control of Pseudomonas aeruginosa infections. PMID: 29198700
  2. The results suggest that trimerization is crucial for both structure and function of general porin OmpF in E. coli, whereas being trimer in substrate-specific channel OprP supports a pore function in Pseudomonas aeruginosa. PMID: 26895142
  3. Study used x-ray crystallography, free-energy molecular dynamics (MD) simulations, and electrophysiology to uncover the atomic basis for the different substrate specificity of OprP and OprO PMID: 26445443
  4. Data indicate the role of the R133 residue - particularly its charge and ability to change the solvation behavior of the permeating ion - in the structure-function relationship of outer membrane porin OprP. PMID: 23875754
Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
OprO/OprP family
Database Links

KEGG: pae:PA3279

STRING: 208964.PA3279

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