ponA Antibody

Code CSB-PA331077ZA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) ponA Polyclonal antibody
Uniprot No.
Target Names
ponA
Alternative Names
Penicillin-binding protein 1A/1B (PBP1) [Includes: Penicillin-insensitive transglycosylase (EC 2.4.1.129) (Peptidoglycan TGase), Penicillin-sensitive transpeptidase (EC 3.4.16.4) (DD-transpeptidase)], ponA
Raised in
Rabbit
Species Reactivity
Bacillus subtilis
Immunogen
Recombinant Bacillus subtilis ponA protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (Probable). Required for vegetative growth (Probable). Has a partially redundant function with PBP-2A (pbpA) during spore outgrowth.
Gene References into Functions
  1. Elimination of PBP1 suppresses the lethality of an mreB null mutation. Polar cell wall bulging is associated with abnormal localization of PBP1. PMID: 19192185
Subcellular Location
Cell membrane; Single-pass type II membrane protein. Forespore inner membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells.
Protein Families
Glycosyltransferase 51 family; Transpeptidase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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