pscF Antibody

Code CSB-PA310982ZA01EZX
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Product Details

Full Product Name
Rabbit anti-Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) pscF Polyclonal antibody
Uniprot No.
Target Names
pscF
Alternative Names
pscF antibody; PA1719 antibody; Type III export protein PscF antibody; Pseudomonas secretion protein F antibody
Raised in
Rabbit
Species Reactivity
Pseudomonas aeruginosa
Immunogen
Recombinant Pseudomonas aeruginosa pscF protein
Immunogen Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Major component of the type III secretion needle structure that plays an essential role in translocation of effector toxins into host cells, facilitating the establishment and dissemination of infection.
Gene References into Functions
  1. PscE and PscG prevent PscF from polymerizing prematurely in the P. aeruginosa cytoplasm and keep it in a secretion prone conformation PMID: 16115870
  2. the P. aeruginosa type III secretory needle structure is composed essentially of PscF, a protein required for secretion and P. aeruginosa cytotoxicity PMID: 16239085
Subcellular Location
Secreted. Cell surface. Note=Secreted via type III secretion system (TTSS).
Protein Families
MxiH/PrgI/YscF family
Database Links

KEGG: pae:PA1719

STRING: 208964.PA1719

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