zipA Antibody

Code CSB-PA303519ZA01ENV
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Product Details

Full Product Name Rabbit anti-Escherichia coli (strain K12) zipA Polyclonal antibody
Uniprot No. P77173
Target Names zipA
Alternative Names zipA antibody; b2412 antibody; JW2404 antibody; Cell division protein ZipA antibody; FtsZ interacting protein A antibody
Raised in Rabbit
Species Reactivity Escherichia coli
Immunogen Recombinant Escherichia coli zipA protein
Immunogen Species Escherichia coli (strain K12)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (12-14 weeks)

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Target Background

(From Uniprot)
Essential cell division protein that stabilizes the FtsZ protofilaments by cross-linking them and that serves as a cytoplasmic membrane anchor for the Z ring. Also required for the recruitment to the septal ring of the downstream cell division proteins FtsK, FtsQ, FtsL and FtsN. ZipA overproduction protects FtsZ from degradation by ClpP by preventing recognition by ClpX. Does not affect the GTPase activity of FtsZ.
Gene References into Functions
  1. study demonstrates a link between amino acid metabolism and ZipA, another essential cell division protein that binds directly to FtsZ and tethers it to the cytoplasmic membrane PMID: 29061666
  2. YciB protein directly interacted with ZipA. Furthermore, the septum localization of ZipA, an essential protein of cell division, is disturbed in a DeltayciB mutant PMID: 26391555
  3. A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells. PMID: 23922320
  4. Data suggest a functional scenario in which ZipA acts as a flexible tether anchoring bacterial proto-ring elements to the membrane during the earlier stages of division. PMID: 23660966
Subcellular Location Cell inner membrane; Single-pass type I membrane protein.
Protein Families ZipA family
Database Links

KEGG: ecj:JW2404

STRING: 316385.ECDH10B_2577

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