zipA Antibody

Code CSB-PA303519ZA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) zipA Polyclonal antibody
Uniprot No.
Target Names
zipA
Alternative Names
zipA antibody; b2412 antibody; JW2404 antibody; Cell division protein ZipA antibody; FtsZ interacting protein A antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli zipA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Essential cell division protein that stabilizes the FtsZ protofilaments by cross-linking them and that serves as a cytoplasmic membrane anchor for the Z ring. Also required for the recruitment to the septal ring of the downstream cell division proteins FtsK, FtsQ, FtsL and FtsN. ZipA overproduction protects FtsZ from degradation by ClpP by preventing recognition by ClpX. Does not affect the GTPase activity of FtsZ.
Gene References into Functions
  1. study demonstrates a link between amino acid metabolism and ZipA, another essential cell division protein that binds directly to FtsZ and tethers it to the cytoplasmic membrane PMID: 29061666
  2. YciB protein directly interacted with ZipA. Furthermore, the septum localization of ZipA, an essential protein of cell division, is disturbed in a DeltayciB mutant PMID: 26391555
  3. A mutation in the promoter region of zipA, a component of the divisome, suppresses the shape defect of RodZ-deficient cells. PMID: 23922320
  4. Data suggest a functional scenario in which ZipA acts as a flexible tether anchoring bacterial proto-ring elements to the membrane during the earlier stages of division. PMID: 23660966
Subcellular Location
Cell inner membrane; Single-pass type I membrane protein.
Protein Families
ZipA family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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