Bovine Beta-Endorphin,β-EP ELISA Kit

Code CSB-E13051B
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation β-EP
Target Name Beta-Endorphin,β-EP
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %90-99
1:2Average %84
Range %80-88
1:4Average %92
Range %88-97
1:8Average %90
Range %85-94
Recovery
The recovery of bovine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9995-104
EDTA plasma (n=4)9288-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30001.701 1.612 1.657 1.559
15001.376 1.458 1.417 1.319
7501.113 1.104 1.109 1.011
3750.832 0.809 0.821 0.723
187.50.524 0.560 0.542 0.444
93.750.367 0.359 0.363 0.265
46.880.264 0.251 0.258 0.160
00.099 0.097 0.098  
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