Bovine Immunoglobulin M,IgM ELISA Kit

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Code CSB-E12017B
Size 96T,5×96T,10×96T
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Product Details

Target Name
Immunoglobulin M
Abbreviation
IgM
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.39 μg/mL-25 μg/mL
Sensitivity
0.2 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 86-99  
1:200 Average % 97  
Range % 92-103  
1:400 Average % 85  
Range % 81-87  
1:800 Average % 89  
Range % 83-95  
Recovery
The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
EDTA plasma (n=4) 97 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
25 0.059 0.056 0.058    
12.5 0.091 0.099 0.095    
6.25 0.151 0.152 0.152    
3.12 0.312 0.292 0.302    
1.56 0.608 0.660 0.634    
0.78 0.824 0.801 0.813    
0.39 1.150 1.192 1.171    
0 1.552 1.508 1.530    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Immunoglobulin M (IgM) is the largest antibody isotype and the first immunoglobulin produced during immune responses. This pentameric molecule serves as the primary antibody in acute infections. It plays an important role in complement activation and agglutination reactions. IgM levels are important indicators of immune system function and are frequently measured in clinical and research settings to assess immune status, monitor disease progression, and evaluate vaccine responses in various species including cattle.

The Bovine Immunoglobulin M ELISA Kit (CSB-E12017B) provides quantitative measurement of IgM in Bos taurus samples using a competitive assay principle. This kit works with serum, plasma, cell culture supernates, and tissue homogenates with a detection range of 0.39-25 μg/mL and sensitivity of 0.2 μg/mL. The assay requires 50-100 μL sample volume, completes within 1-5 hours, and uses detection at 450 nm wavelength for reliable quantification of bovine IgM levels.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in bovine research studies to measure immunoglobulin M levels as part of comprehensive immune response studies. The kit supports research examining immune function alongside other immunoglobulins and inflammatory markers in cattle.

Immune profiling studies: Used to quantify IgM concentrations in serum samples as part of broader immunological evaluations that include multiple immunoglobulin classes and cytokines
Antioxidant and immune response research: Applied in studies examining the relationship between oxidative stress markers and immune system parameters in bovine subjects
Plasma immune marker analysis: Used for measuring IgM levels in plasma samples alongside other immune indices including various interleukins and immunoglobulin classes

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