Human Immunoglobulin M,IgM ELISA Kit

Code CSB-E07976h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgM
Target Name Immunoglobulin M,IgM
Alias N/A
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 4.68 ng/mL-300 ng/mL
Sensitivity 1.17 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5000 Average % 86  
Range % 80-94  
1:10000 Average % 97  
Range % 91-105  
1:20000 Average % 95  
Range % 91-102  
1:40000 Average % 94  
Range % 86-101  
The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-101  
EDTA plasma (n=4) 97 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
300 2.753 2.769 2.761 2.641  
150 2.404 2.428 2.416 2.296  
75 1.905 1.911 1.908 1.788  
37.5 1.281 1.293 1.287 1.167  
18.75 0.726 0.729 0.728 0.608  
9.37 0.465 0.472 0.469 0.349  
4.68 0.288 0.294 0.291 0.171  
0 0.121 0.119 0.120    
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