Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit

Code CSB-E17548B
Size 96T,5×96T,10×96T
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Product Details

Target Name
Thyroid Stimulating Hormone(TSH)
Alternative Names
TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation
TSH
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.9 uIU/mL-60 uIU/mL
Sensitivity
0.225 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %88
Range %84-92
1:4Average %90
Range %85-98
1:8Average %92
Range %88-96
Recovery
The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-104
EDTA plasma (n=4)8480-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µIU/ml OD1OD2AverageCorrected
601.815 1.731 1.773 1.626
301.279 1.237 1.258 1.111
150.934 0.927 0.931 0.784
7.50.623 0.671 0.647 0.500
3.750.408 0.379 0.394 0.247
1.80.315 0.297 0.306 0.159
0.90.201 0.195 0.198 0.051
00.150 0.143 0.147
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine TSH ELISA Kit was designed for the quantitative measurement of Bovine TSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 uIU/mL-60 uIU/mL and the sensitivity is 0.225 μIU/mL.

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Target Background

Function
(From Uniprot)
Indispensable for the control of thyroid structure and metabolism.
Gene References into Functions
  1. Nitric oxide decreases TSH-induced FoxE1 expression, thus repressing the transcripcional activation of TPO gene. PMID: 26610751
  2. identification for the first time that ADAM10 is a TSHR cleavage enzyme and demonstration that TSH regulates its activation PMID: 18074395
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Database Links

KEGG: bta:281552

STRING: 9913.ENSBTAP00000008264

UniGene: Bt.475

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