Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit

Instructions
Code CSB-E17548B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Thyroid Stimulating Hormone(TSH)
Alternative Names TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation TSH
Uniprot No. P01223
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.9 uIU/mL-60 uIU/mL
Sensitivity 0.225 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %88
Range %84-92
1:4Average %90
Range %85-98
1:8Average %92
Range %88-96
Recovery
The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-104
EDTA plasma (n=4)8480-88
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
µIU/ml OD1OD2AverageCorrected
601.815 1.731 1.773 1.626
301.279 1.237 1.258 1.111
150.934 0.927 0.931 0.784
7.50.623 0.671 0.647 0.500
3.750.408 0.379 0.394 0.247
1.80.315 0.297 0.306 0.159
0.90.201 0.195 0.198 0.051
00.150 0.143 0.147
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Indispensable for the control of thyroid structure and metabolism.
Gene References into Functions
  1. Nitric oxide decreases TSH-induced FoxE1 expression, thus repressing the transcripcional activation of TPO gene. PMID: 26610751
  2. identification for the first time that ADAM10 is a TSHR cleavage enzyme and demonstration that TSH regulates its activation PMID: 18074395
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: bta:281552

STRING: 9913.ENSBTAP00000008264

UniGene: Bt.475

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