Bovine Trypsin ELISA Kit

Bovine Trypsin ELISA Kit

Product Details

Customer Reviews and Q&A

Product Details

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Customer Reviews and Q&A

Protocol

Code	CSB-EL018811BO
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Target Name

Trypsin

Abbreviation

Trypsin

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	87
	Range %	82-94
1:800	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

4000

0.180

0.174

0.177

2000

0.275

0.267

0.271

1000

0.429

0.420

0.424

500

0.613

0.629

0.621

250

0.985

1.021

1.003

125

1.581

1.653

1.617

62.5

2.093

2.184

2.139

2.652

2.725

2.688

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Bovine Trypsin ELISA Kit was designed for the quantitative measurement of Bovine Trypsin protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.