Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Code CSB-E16370B
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
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Product Details

Target Name
lipopolysaccharide binding protein
Alternative Names
LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
Abbreviation
LBP
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 84-102  
1:2 Average % 86  
Range % 82-95  
1:4 Average % 98  
Range % 90-107  
1:8 Average % 95  
Range % 90-100  
Recovery
The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 95 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.126 2.150 2.138 2.002  
500 1.611 1.623 1.617 1.481  
250 1.142 1.158 1.150 1.014  
125 0.797 0.780 0.789 0.653  
62.5 0.463 0.451 0.457 0.321  
31.25 0.340 0.334 0.337 0.201  
15.6 0.271 0.275 0.273 0.137  
0 0.135 0.136 0.136    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine LBP ELISA Kit was designed for the quantitative measurement of Bovine LBP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

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 Q&A
Q:

I am interested in the product CSB-E16370B and would like to confirm:
Has this kit actually been validated with bovine samples, or is it based off a sequence homology?

A:
Thanks for your inquiry.
We tested bovine serum and plasma samples before and the test value is 15.6-62.5ng/ml.
We suggest you use the kit for the corresponding species to test your samples.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Gene References into Functions
  1. Our study provides an overview of the gene expression profile between wild LBP and mutant LBP on the LPS-induced inflammatory response of bovine mammary epithelial cells , which will lead to further understanding of the potential effects of LBP mutations on bovine mammary glands. PMID: 26813383
  2. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
  3. One possible anti-inflammatory mechanism can be attributed to the negative role of BRLBP in suppressing TLR4/NF-kappaB activation mediated by LPS. These findings suggested that BRLBP may be a useful agent to treat LPS-induced mastitis PMID: 25700343
  4. Single nucleotide polymorphisms in the lipopolysaccharide-binding protein may hold the secret of susceptibility to clinical mastitis in Chinese Holstein. PMID: 22733492
Subcellular Location
Secreted. Cytoplasmic granule membrane.
Protein Families
BPI/LBP/Plunc superfamily, BPI/LBP family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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