Bovine lipopolysaccharide binding protein (LBP) ELISA kit

Instructions
Code CSB-E16370B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lipopolysaccharide binding protein
Alternative Names LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
Abbreviation LBP
Uniprot No. Q2TBI0
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 84-102  
1:2 Average % 86  
Range % 82-95  
1:4 Average % 98  
Range % 90-107  
1:8 Average % 95  
Range % 90-100  
Recovery
The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 95 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.126 2.150 2.138 2.002  
500 1.611 1.623 1.617 1.481  
250 1.142 1.158 1.150 1.014  
125 0.797 0.780 0.789 0.653  
62.5 0.463 0.451 0.457 0.321  
31.25 0.340 0.334 0.337 0.201  
15.6 0.271 0.275 0.273 0.137  
0 0.135 0.136 0.136    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

I am interested in the product CSB-E16370B and would like to confirm:
Has this kit actually been validated with bovine samples, or is it based off a sequence homology?

A:
Thanks for your inquiry.
We tested bovine serum and plasma samples before and the test value is 15.6-62.5ng/ml.
We suggest you use the kit for the corresponding species to test your samples.
Pls let me know if you have any further questions. Thank you.
 Customer Reviews

Target Data

Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Gene References into Functions
  1. Our study provides an overview of the gene expression profile between wild LBP and mutant LBP on the LPS-induced inflammatory response of bovine mammary epithelial cells , which will lead to further understanding of the potential effects of LBP mutations on bovine mammary glands. PMID: 26813383
  2. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
  3. One possible anti-inflammatory mechanism can be attributed to the negative role of BRLBP in suppressing TLR4/NF-kappaB activation mediated by LPS. These findings suggested that BRLBP may be a useful agent to treat LPS-induced mastitis PMID: 25700343
  4. Single nucleotide polymorphisms in the lipopolysaccharide-binding protein may hold the secret of susceptibility to clinical mastitis in Chinese Holstein. PMID: 22733492
Subcellular Location Secreted, Cytoplasmic granule membrane
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Database Links

KEGG: bta:512242

STRING: 9913.ENSBTAP00000022428

UniGene: Bt.24181

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