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Canine Free Thyroxine,FT4 ELISA Kit

Canine Free Thyroxine,FT4 ELISA Kit

Protocol

Code	CSB-E13196c
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details

Target Name

Free Thyroxine,FT4

Abbreviation

FT4

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.5 ng/mL-120 ng/mL

Sensitivity

7.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-97
1:2	Average %	90
	Range %	88-100
1:4	Average %	95
	Range %	90-105
1:8	Average %	91
	Range %	85-102

Recovery

The recovery of canine FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-105
EDTA plasma (n=4)	95	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
120	0.151	0.148	0.150
60	0.280	0.283	0.282
30	0.455	0.462	0.459
15	0.869	0.861	0.865
7.5	1.823	1.860	1.842

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Dog FT4 ELISA Kit was designed for the quantitative measurement of Dog FT4 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 7.5 ng/mL-120 ng/mL and the sensitivity is 7.5 ng/mL.

Customer Reviews and Q&A

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■ Q&A

Could you kindly let me is it of 100% canine specific?

Thanks for your inquiry. It is 100% specific to canine .Though T4 is small molecule and it is the same among different species.But as there is matrix difference among different species ,we develop different kits for different species. Pls let me know if you have any further questions. Thank you.

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Canine Free Thyroxine,FT4 ELISA Kit

Product Details

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Customer Reviews and Q&A