Mouse Free Thyroxine,FT4 ELISA Kit

Free thyroxine (FT4) represents the unbound, metabolically active fraction of thyroxine hormone in circulation, constituting approximately 0.03% of total thyroid hormone. As the primary hormone produced by the thyroid gland, FT4 regulates cellular metabolism, growth, and development throughout the body. Unlike total thyroxine measurements, FT4 levels provide a more accurate picture of thyroid function since they remain unaffected by variations in thyroid-binding proteins. This makes FT4 an important biomarker in endocrine research and thyroid disorder studies.

The Mouse Free Thyroxine FT4 ELISA Kit (CSB-E05080m) measures FT4 concentrations in mouse serum, plasma, and tissue homogenates. This competitive assay operates within a detection range of 4-64 pmol/L with a sensitivity of 1.08 pmol/L. The kit requires 50-100 μL sample volume and provides results within 1-5 hours using spectrophotometric detection at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining thyroid hormone regulation and endocrine function in mouse models. Studies have applied the kit to measure free thyroxine levels in serum samples as part of broader hormonal profiling approaches. The kit supports research examining metabolic and endocrine pathways where thyroid hormone status serves as a key parameter.

• Endocrine profiling - Used alongside other hormone measurements to determine thyroid function status in experimental studies
• Metabolic research - Applied in studies examining relationships between thyroid hormones and metabolic parameters including lipid profiles
• Stress response studies - Used to evaluate thyroid hormone levels in conjunction with stress-related hormones in serum samples
• Comprehensive hormone panels - Integrated into multi-analyte approaches measuring various endocrine markers simultaneously