Target Name
Matrix metalloproteinase 1,MMP-1
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.18 ng/mL-12 ng/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:1 | Average % | 93 | |
Range % | 85-102 | |
1:2 | Average % | 89 | |
Range % | 85-99 | |
1:4 | Average % | 94 | |
Range % | 85-97 | |
1:8 | Average % | 90 | |
Range % | 83-98 | |
Recovery
The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 94 | 82-98 | |
EDTA plasma (n=4) | 97 | 89-100 | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
ng/ml | OD1 | OD2 | Average | Corrected | | 12 | 2.309 | 2.326 | 2.318 | 2.114 | | 6 | 1.754 | 1.699 | 1.727 | 1.523 | | 3 | 1.301 | 1.338 | 1.320 | 1.116 | | 1.5 | 0.931 | 0.920 | 0.926 | 0.722 | | 0.75 | 0.573 | 0.585 | 0.579 | 0.375 | | 0.37 | 0.412 | 0.407 | 0.410 | 0.206 | | 0.18 | 0.299 | 0.303 | 0.301 | 0.097 | | 0 | 0.201 | 0.206 | 0.204 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Dog MMP-1 ELISA Kit was designed for the quantitative measurement of Dog MMP-1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.04 ng/mL.