Target Name
matrix metalloproteinase 1,MMP-1
Alternative Names
Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
47 pg/mL-3000 pg/mL
Detection Wavelength
450 nm
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:20 | Average % | 91 | |
| Range % | 86-95 | |
| 1:40 | Average % | 100 | |
| Range % | 97-103 | |
| 1:80 | Average % | 91 | |
| Range % | 87-95 | |
| 1:160 | Average % | 97 | |
| Range % | 93-100 | |
Recovery
| The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-97 | |
| EDTA plasma (n=4) | 96 | 90-98 | |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 2.482 | 2.510 | 2.496 | 2.317 | | | 1500 | 1.855 | 1.786 | 1.821 | 1.642 | | | 750 | 1.246 | 1.232 | 1.239 | 1.060 | | | 375 | 0.748 | 0.774 | 0.761 | 0.582 | | | 187.5 | 0.495 | 0.456 | 0.476 | 0.297 | | | 93.75 | 0.322 | 0.339 | 0.331 | 0.152 | | | 46.88 | 0.253 | 0.242 | 0.248 | 0.069 | | | 0 | 0.180 | 0.178 | 0.179 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
