Target Name
Thyroid Stimulating Hormone,TSH
Alternative Names
TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 μIU/mL-10 μIU/mL
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of canine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-98 |
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| 1:2 |
Average % |
95 |
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| Range % |
92-100 |
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| 1:4 |
Average % |
82 |
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| Range % |
80-84 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-99 |
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Recovery
| The recovery of canine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-95 |
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| EDTA plasma (n=4) |
93 |
90-96 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| μIU/ml |
OD1 |
OD2 |
Average |
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| 10 |
0.195 |
0.188 |
0.192 |
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| 5 |
0.334 |
0.353 |
0.344 |
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| 2.5 |
0.531 |
0.514 |
0.523 |
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| 1.25 |
1.017 |
1.102 |
1.060 |
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| 0.625 |
2.138 |
2.320 |
2.229 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Dog TSH ELISA Kit was designed for the quantitative measurement of Dog TSH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.625 μIU/mL-10 μIU/mL and the sensitivity is 0.625 μIU/mL.
