| Description |
This Chicken IL1B ELISA Kit was designed for the quantitative measurement of Chicken IL1B protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.27 pg/mL-200 pg/mL and the sensitivity is 0.27 pg/mL. |
| Target Name |
Interleukin 1β,IL-1β |
| Abbreviation |
IL1B |
| Uniprot No. |
O73909 |
| Species |
Gallus gallus (Chicken) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.27 pg/mL-200 pg/mL |
| Sensitivity |
0.27 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology
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| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
|
| Range % |
90-100 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-106 |
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| 1:4 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of chicken IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-98 |
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| EDTA plasma (n=4) |
95 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
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| 200 |
0.173 |
0.169 |
0.171 |
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| 66.7 |
0.238 |
0.259 |
0.249 |
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| 22.2 |
0.339 |
0.367 |
0.353 |
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| 7.4 |
0.459 |
0.488 |
0.474 |
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| 2.5 |
0.602 |
0.633 |
0.618 |
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| 0.82 |
0.817 |
0.855 |
0.836 |
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| 0.27 |
1.033 |
1.008 |
1.021 |
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| 0 |
1.748 |
1.672 |
1.710 |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
