Chicken Interleukin 1β,IL-1β ELISA Kit

Code CSB-E11230Ch
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Chicken IL1B ELISA Kit was designed for the quantitative measurement of Chicken IL1B protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.27 pg/mL-200 pg/mL and the sensitivity is 0.27 pg/mL.

Target Name Interleukin 1β,IL-1β
Abbreviation IL1B
Uniprot No. O73909
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.27 pg/mL-200 pg/mL
Sensitivity 0.27 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-100  
1:2 Average % 99  
Range % 95-106  
1:4 Average % 84  
Range % 80-88  
1:8 Average % 93  
Range % 86-98  
The recovery of chicken IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-98  
EDTA plasma (n=4) 95 90-99  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
200 0.173 0.169 0.171    
66.7 0.238 0.259 0.249    
22.2 0.339 0.367 0.353    
7.4 0.459 0.488 0.474    
2.5 0.602 0.633 0.618    
0.82 0.817 0.855 0.836    
0.27 1.033 1.008 1.021    
0 1.748 1.672 1.710    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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