Dog Complement 4(C4) ELISA kit

Instructions
Code CSB-EQ027970DO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Complement 4(C4)
Abbreviation C4
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.8 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 97  
Range % 92-105  
1:400 Average % 96  
Range % 92-99  
1:800 Average % 103  
Range % 98-109  
1:1600 Average % 87  
Range % 82-91  
Recovery
The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 88-98  
EDTA plasma (n=4) 90 87-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2000 0.080 0.079 0.080    
1000 0.117 0.125 0.121    
500 0.202 0.198 0.200    
250 0.311 0.305 0.308    
125 0.499 0.513 0.506    
62.5 0.927 0.933 0.930    
31.25 1.457 1.342 1.400    
0 2.559 2.438 2.499    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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