Dog Complement 4(C4) ELISA kit

Dog Complement 4(C4) ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Dog Complement 4(C4) ELISA kit

Citations (1) Protocol

Code	CSB-EQ027970DO
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (1)
Related Products
Customer Reviews and Q&A

Target Name

Complement 4(C4)

Abbreviation

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 ng/mL-2000 ng/mL

Sensitivity

7.8 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	96
	Range %	92-99
1:800	Average %	103
	Range %	98-109
1:1600	Average %	87
	Range %	82-91

Recovery

The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	90	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.080	0.079	0.080
1000	0.117	0.125	0.121
500	0.202	0.198	0.200
250	0.311	0.305	0.308
125	0.499	0.513	0.506
62.5	0.927	0.933	0.930
31.25	1.457	1.342	1.400
0	2.559	2.438	2.499

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Dog Complement 4 (C4) ELISA Kit was designed for the quantitative measurement of Dog Complement 4 (C4) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL .

Serum concentrations of complement C3 and C4 in dogs with idiopathic epilepsy J Zhou,Journal of veterinary internal medicine,2024

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Dog

Sample dilution: : 1:9

Review: I used the canine complement C4 enzyme-linked immunosorbent assay kit to detect the C4 content in canine serum, and the results were good. The standard curve showed good linearity, the RSD of replicate wells was less than 15%, and the concentrations of diluted samples were all within the linear range. The standard curves of the three indicator kits were calculated using LOG-LOG simulation.

By Anonymous

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.