Dog growth hormone,GH ELISA Kit

Dog growth hormone,GH ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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Dog growth hormone,GH ELISA Kit

Citations (1) Protocol MSDS

Code	CSB-E14962c
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (1)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

growth hormone 1

Alternative Names

GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit

Abbreviation

GH1

Uniprot No.

P33711

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	98
	Range %	91-105
1:4	Average %	89
	Range %	84-95
1:8	Average %	99
	Range %	92-106

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.853

2.734

2.794

2.632

2.087

2.067

2.077

1.916

1.504

1.498

1.501

1.339

0.897

0.929

0.913

0.751

2.5

0.550

0.521

0.536

0.374

1.25

0.383

0.363

0.373

0.211

0.625

0.271

0.284

0.277

0.116

0.158

0.165

0.162

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Dog GH1 ELISA Kit was designed for the quantitative measurement of Dog GH1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Age-specific variations in hematological and biochemical parameters in middle-and large-sized of dogs SH Lee,J Vet Sci,2019

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Function
(From Uniprot)

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Gene References into Functions

Progesterone alone has no direct stimulatory effect on GH transcription in mammary gland. PMID: 18262383
Study provides evidence that progesteron might increase autocrine Growth Hormone production which might directly stimulate local or systemic IGF-I secretion in canine mammary tumours. PMID: 18367392

Subcellular Location

Secreted.

Protein Families

Somatotropin/prolactin family

Database Links

KEGG: cfa:403795

STRING: 9615.ENSCAFP00000018659

UniGene: Cfa.3656

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Jak-STAT signaling pathway PI3K-Akt signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.