Fish Catalase (CAT) ELISA kit

Code CSB-E15928Fh
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Catalase (CAT)
Abbreviation
Uniprot No.
Species
Fish
Sample Types
serum, plasma
Detection Range
125 mU/mL-2000 mU/mL
Sensitivity
62.5 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 91-99  
1:200 Average % 103  
Range % 99-107  
1:400 Average % 98  
Range % 93-105  
1:800 Average % 92  
Range % 87-95  
Recovery
The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-104  
EDTA plasma (n=4) 88 83-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average    
2000 0.091 0.089 0.090    
1000 0.242 0.257 0.250    
500 0.553 0.587 0.570    
250 0.913 0.980 0.947    
125 1.647 1.563 1.605    
0 2.381 2.319 2.350    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Fish CAT ELISA Kit was designed for the quantitative measurement of Fish CAT protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 125 mU/mL-2000 mU/mL and the sensitivity is 62.5 mU/mL.

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