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Fish Catalase (CAT) ELISA kit

Instructions
Code CSB-E15928Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Catalase (CAT)
Abbreviation CAT
Uniprot No. H2L8K1
Species Fish
Sample Types serum, plasma
Detection Range 125 mU/mL-2000 mU/mL
Sensitivity 62.5 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 91-99  
1:200 Average % 103  
Range % 99-107  
1:400 Average % 98  
Range % 93-105  
1:800 Average % 92  
Range % 87-95  
Recovery
The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-104  
EDTA plasma (n=4) 88 83-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average    
2000 0.091 0.089 0.090    
1000 0.242 0.257 0.250    
500 0.553 0.587 0.570    
250 0.913 0.980 0.947    
125 1.647 1.563 1.605    
0 2.381 2.319 2.350    
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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