Target Name |
Glutathione Peroxidase (GSH-PX) |
Abbreviation |
GSH-PX |
Species |
Fish |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
375 μU/mL-6000 μU/mL |
Sensitivity |
187.5 μU/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Metabolism
|
Assay Principle |
quantitative |
Measurement |
Competitive |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
1:100 |
Average % |
95 |
|
Range % |
91-99 |
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1:200 |
Average % |
103 |
|
Range % |
99-107 |
|
1:400 |
Average % |
98 |
|
Range % |
93-103 |
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1:800 |
Average % |
92 |
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Range % |
87-97 |
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Recovery |
The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
|
Serum (n=5) |
99 |
94-104 |
|
EDTA plasma (n=4) |
89 |
84-94 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
μU/ml |
OD1 |
OD2 |
Average |
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6000 |
0.089 |
0.084 |
0.087 |
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3000 |
0.246 |
0.265 |
0.256 |
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1500 |
0.551 |
0.580 |
0.566 |
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750 |
0.933 |
0.981 |
0.957 |
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375 |
1.646 |
1.569 |
1.608 |
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0 |
2.362 |
2.398 |
2.380 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |