Fish Glutathione Peroxidase (GSH-PX) ELISA kit

Instructions
Code CSB-E15930Fh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Glutathione Peroxidase (GSH-PX)
Abbreviation GSH-PX
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 375 μU/mL-6000 μU/mL
Sensitivity 187.5 μU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 91-99  
1:200 Average % 103  
Range % 99-107  
1:400 Average % 98  
Range % 93-103  
1:800 Average % 92  
Range % 87-97  
Recovery
The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 99 94-104  
EDTA plasma (n=4) 89 84-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μU/ml OD1 OD2 Average    
6000 0.089 0.084 0.087    
3000 0.246 0.265 0.256    
1500 0.551 0.580 0.566    
750 0.933 0.981 0.957    
375 1.646 1.569 1.608    
0 2.362 2.398 2.380    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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