Interleukin 1β (IL-1β) is a pro-inflammatory cytokine that plays a central role in immune responses and inflammatory processes across vertebrate species. Activated macrophages and monocytes produce this cytokine in response to various stimuli, including pathogens and tissue damage. IL-1β acts as a key mediator in the initiation and amplification of inflammatory cascades, promoting the recruitment and activation of immune cells. In fish immunology research, IL-1β serves as an important biomarker for studying innate immune responses, disease susceptibility, and the effects of environmental stressors on aquatic organisms.
The Fish Interleukin 1β (IL-1β) ELISA Kit (CSB-E13259Fh) is a quantitative sandwich assay designed for measuring IL-1β levels in fish samples. This kit works with serum, plasma, and tissue homogenates with a detection range of 3.12 pg/mL to 200 pg/mL and a sensitivity of 0.78 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for IL-1β quantification in fish immunological studies.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in fish research to quantify interleukin 1β levels as part of comprehensive immune response studies. The kit supports research examining inflammatory biomarkers in aquatic species, particularly in the context of stress response monitoring and immune system analysis.
• Immune response profiling: Measuring IL-1β levels alongside other immune parameters including lysozyme activity, immunoglobulin levels, and antioxidant enzyme activities in fish plasma samples
• Stress response monitoring: Analyzing inflammatory markers in conjunction with metabolic parameters such as liver enzymes, lipid profiles, and glucose levels following experimental interventions
• Whole-body cytokine analysis: Quantifying mature IL-1β concentrations in complete fish specimens to determine systemic inflammatory responses
• Comparative immunology studies: Examining pro-inflammatory cytokine levels as part of broader biochemical and physiological studies in aquaculture and fish biology research
