Fish immunoglobulin M (IgM) ELISA Kit

Code CSB-E12045Fh
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgM
Target Name immunoglobulin M
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 μg/mL-50 μg/mL
Sensitivity 1.25 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-101  
1:2 Average % 97  
Range % 90-104  
1:4 Average % 95  
Range % 84-105  
1:8 Average % 95  
Range % 91-99  
Recovery
The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 92-105  
EDTA plasma (n=4) 95 91-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
50 0.122 0.124 0.123    
25 0.213 0.199 0.206    
11.5 0.401 0.427 0.414    
3.75 0.745 0.761 0.753    
1.25 1.375 1.402 1.389    
0 2.541 2.532 2.537    
References
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Q&A and Customer Reviews

Q&A
Q:

CUSABIO fish immnoglobulin M (IgM) ELISA kit Does this kit can be used for separate experiments?around 30-40 wells every time?

A:
Thanks for your inquiry.
#CSB-E12045Fh,Fish immunoglobulin M (IgM) ELISA Kit
YES,it can.The plate is Breakapart and can be used for seperate experiments.You can put the break part in the
Aluminum foil bag for next use.The manual is provided to you by e-mail.Additionally,we received 21 published papers until now.You can refer to it. Pls let me know if you have any further questions. Thank you.
Q:

please provide the following information regarding Fish immunoglobulin M, IgM ELISA Kit (CSB-E12045Fh)
1. What is the standard?
2. What is the antibody in this kit?

A:
Thanks for your inquiry.
CSB-E12045Fh
Standard is purified crude extract of natural samples. The plate is coated with goat anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody. The immunogen is purified crude extract of natural samples.
Pls let me know if you have any further questions. Thank you.
Q:

Regarding your ELISA kit CSB-E12045Fh, could you please advise on the following:
1. Capture Antibody: host animal species, clonality
2. Detection Antibody: host animal species, clonality
3. Standard
Also, what sample types have been tested with this kit, and what were the reference values?

A:
Thanks for your inquiry.
CSB-E12045Fh
The target is a special protein and there is no uniprot.
This kit is based on competitive method. The plate is coated with goat-anti-rabbit antibody. The specific antibody is rabbit polyclonal antibody. The standard is purified crude extract of natural samples.
We tested undiluted serum and plasma before and the test value is 1.25--3.75μg/ml.
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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