Fish immunoglobulin M (IgM) ELISA Kit

The fish immunoglobulin M (IgM) ELISA kit is suitable for the quantitative measurement of the IgM levels in fish serum, plasma, or tissue homogenates. It provides a detection range of 1.25 μg/mL-50 μg/mL. It also has high sensitivity, excellent specificity, high precision, good linearity, and high recovery.

This assay employs the competitive inhibition enzyme immunoassay technique using an anti-fish IgM antibody and an HRP-conjugated IgM. In this assay, the microplate has been pre-coated with a goat-anti-rabbit antibody. Samples or standards are pipetted into wells with the anti-fish IgM antibody and HRP-conjugated IgM. IgM in the samples or the standards and HRP-conjugated IgM competes with binding to the anti-fish IgM antibody. The solution appears blue after the addition of the TMB substrate. The color reaction is terminated after adding the stop solution. The color intensity is inverse to the amount of IgM in the sample.

IgM is the initial antibody produced by B cells in response to a foreign pathogen. It is rapidly generated during a primary immune response. It usually lasts for about 3 weeks, peaks in the second week, and declines gradually till it disappears. IgG emerges at this time. IgM not only acts as the first line of host defense against infections but also plays an important role in immune modulation and immunological tolerance.