Fish immunoglobulin M (IgM) ELISA Kit

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Fish immunoglobulin M (IgM) ELISA Kit

Name: Fish immunoglobulin M (IgM) ELISA Kit
Brand: CUSABIO
SKU: CSB-E12045Fh

^Instructions

Code	CSB-E12045Fh
Size	96T,5×96T,10×96T
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Target Name

immunoglobulin M

Abbreviation

IgM

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 μg/mL-50 μg/mL

Sensitivity

1.25 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-101
1:2	Average %	97
	Range %	90-104
1:4	Average %	95
	Range %	84-105
1:8	Average %	95
	Range %	91-99

Recovery

The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	95	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
50	0.122	0.124	0.123
25	0.213	0.199	0.206
11.5	0.401	0.427	0.414
3.75	0.745	0.761	0.753
1.25	1.375	1.402	1.389
0	2.541	2.532	2.537

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Impact of Tilapia hepcidin 2-3 dietary supplementation on the gut microbiota profile and immunomodulation in the grouper (Epinephelus lanceolatus) Ting C H, et al,Scientific Reports,2019
EFFECT OF PLANT BIOPSIES LACTOBACILLUS PLANTARUM VSG3 IMPROVES ON IMPROVE RESISTANCE OF FRESHWATER FISH TO DISEASES Shokr E A M, et al,Abbassa Int. J. Aqua,2019
Dietary supplementation of recombinant tilapia piscidin 4-expressing yeast enhances growth and immune response in Lates calcarifer Han-Ning Huang, et al,Aquaculture Reports,2019
EFFECT OF GINGER ON SOME HEMATOLOGICAL ASPECTS AND IMMUNE SYSTEM IN NILE TILAPIA Shokr E A M, et al,Abbassa Int. J. Aqua,2019
Effect of dietary Moringa oleifera leaf on the immune response and control of Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus) fry El-Gawad E A A, et al,Aquaculture International,2019
Modulatory effects of fenugreek seeds powder on the histopathology, oxidative status, and immune related gene expression in Nile tilapia (Oreochromis niloticus) infected with Aeromonas hydrophila Moustafa E M, et al,Aquaculture,2019
Immune-protective, antioxidant and relative genes expression impacts of β-glucan against fipronil toxicity in Nile tilapia, Oreochromis niloticus El Hakim Y A, et al,Fish & Shellfish Immunology,2019
The potential antimicrobial effects of dietary supplementation with Arak, Salvadora persica, on growth, health status, and pathogenic bacterial loads in Nile tilapia, Oreochromis niloticus fingerlings Mohamed M. Toutou, et al,Egyptian Journal of Aquatic Research,2019
The optimal arginine requirement in diets for juvenile humpback grouper, Cromileptes altivelis Wei Mu, et al,Aquaculture,2019
Evaluation of the potential probiotic Bacillus subtilis isolated from two ancient sturgeons on growth performance, serum immunity and disease resistance of Acipenser dabryanus Jun Di, et al,Fish & Shellfish Immunology,2019
Effect of dietary phenylalanine level on growth performance, body composition, and biochemical parameters in plasma of juvenile hybrid tilapia, Oreochromis niloticus × Oreochromis aureus Wei Xiao, et al,Journal of The World Aquaculture Society,2019
Influences of diets supplemented with pomegranate peel on haematology, blood biochemistry and immune status in monosex Nile tilapia, Oreochromis niloticus Ahmed E. A. Badrey,Egyptian Journal of Aquatic Biology & Fisheries,2019
Effects of feeding regimen of dietary Aspergillus oryzae on the growth performance, intestinal morphometry and blood profile of Nile tilapia (Oreochromis niloticus) Mahmoud A. O. Dawood,Aquaculture Nutrition,2019
DIGESTIVE ENZYMES, IMMUNITY AND OXIDATIVE STATUS OF NILE TILAPIA (Oreochromis niloticus) REARED IN INTENSIVE CONDITIONS Mahmoud A Dawood, et al,/,2019
Association of MHC IIA polymorphisms with disease resistance in Aeromonas hydrophila-challenged Nile tilapia El-Magd MA, et al,Developmental & comparative immunology,2019
Modulation of digestive enzyme activity, blood health, oxidative responses and growth-related gene expression in GIFT by heat-killed Lactobacillus plantarum (L-137) Mahmoud A.O.Dawood, et al,Aquaculture,2019
Traditional Chinese Medicine Enhances Growth, Immune Response, and Resistance to Streptococcus agalactiae in Nile Tilapia Abarike ED, et al,Journal Of Aquatic Animal Health,2019
Effect of Dietary Tryptophan Levels on Growth and Hemato-Immunological Parameters in Juvenile Nile Tilapia Oreochromis niloticus Wu Y, et al,The Israeli Journal of Aquaculture - Bamidgeh,2018
EVALUATION OF SPIRULINA PLATENSIS ENRICHED DIET ON GROWTH PERFORMANCE, BIOCHEMICAL PARAMETERS AND IMMUNE RESPONSE OF OREOCHROMIS NILOTICUS Abd-El Alim F Abd-El Alim, et al,Slovenian Veterinary Research,2018
Dietary propionic acid enhances antibacterial and immunomodulatory effects of oxytetracycline on Nile tilapia, Oreochromis niloticus El-Adawy M.et al,Environmental Science and Pollution Research,2018
Effects of dietary yeast culture on growth performance, immune response and disease resistance of gibel carp (Carassius auratus gibelio CAS Ⅲ) Peiyu Zhang .et al,Fish & Shellfish Immunology,2018
Effects of a commercial probiotic BS containing Bacillus subtilis and Bacillus licheniformis on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus Emmanuel Delwin Abarike .et al,Fish & Shellfish Immunology,2018
Effect of dietary yeast nucleotide on antioxidant activity, non-specific immunity, intestinal cytokines, and disease resistance in Nile Tilapia Rasha M.et al,Fish & Shellfish Immunology,2018
Comparative study of three predominant gut Bacillus strains and a commercial B. amyloliquefaciens as probiotics on the performance of Clarias gariepinus Rasha M.Reda.et al,Fish & Shellfish Immunology,2018
Evaluation of In Vivo Probiotic Efficiency of Bacillus amyloliquefaciens in Labeo rohita Challenged by Pathogenic Strain of Aeromonas hydrophila MTCC 1739 Nandi A.et al,Probiotics Antimicrob Proteins,2017
Supplementation of heat-inactivated Bacillus clausii DE5 in diets for grouper, Epinephelus coioides, improves feed utilization, intestinal and systemic immune responses and not growth performance J. Wang.et al,Aquaculture Nutrition,2017
Effects of potential probiotic Bacillus subtilisKADR1 and its subcellular components on immune responses and disease resistance in Labeo rohita Dharmaraj Ramesh.et al,Aquaculture Research,2017
Probiotic efficiency of Bacillus sp. in Labeo rohita challenged by Aeromonas hydrophila: assessment of stress profile, haemato-biochemical parameters and immune responses Ankita Nandi.et al,aquaculture research ,2017
The Effect of Kefir as a Dietary Supplement on Nonspecific Immune Response and Disease Resistance in Juvenile Rainbow Trout, Oncorhynchus mykiss (Walbaum 1792) Uluky G.et al,Journal of the World Aquaculture Society,2016
Effect of Oyster Mushroom, Pleurotus ostreatus, Extract on Hemato-Immunological Parameters of Rainbow Trout, Oncorhynchus mykiss Uluky G.et al,Journal of the World Aquaculture Society,2016
Effects of melatonin and green-wavelength LED light on the physiological stress and immunity of goldfish, Carassius auratus, exposed to high water temperature Jung SJ.et al,Fish Physiol Biochem.,2016
Enhancement of Antioxidant Activity, Non-specific Immunity and Growth Performance of Nile Tilapia, Oreochromis Niloticus by Dietary Fructooligosaccharide Eman A Abd El-Gawad1.et al,J Aquac Res Development,,2016
Influence of diets supplemented with pomegranate peel extract on performance in Oreochromus niloticus Badawi M E.et al,Japanese Journal of Veterinary Research,2016
Enhanced NK cell adoptive antitumor effects against breast cancer in vitro via blockade of the transforming growth factor-ß signaling pathway Zhao Y. et al,Onco Targets Ther,2015
Effects of dietary Persian oak (Quercus brantii var. persica) fruit extract on survival, growth performance, haematological and immunological parameters in rainbow trout, Oncorhynchus mykiss, fingerlings S. Bohlouli et al,Aquaculture Nutrition,2015
Effects of dietary protein levels on growth performance and haemato-immunological parameters of juvenile genetically improved farmed tilapia (GIFT), Oreochromis niloticus Mathew Didlyn Kpundeh et al,Aquaculture International,2015
Early steps in the European eel (Anguilla anguilla)-Vibrio vulnificus interaction in the gills: role of the RtxA13 toxin. Callol A.et al,Fish Shellfish Immunol.,2015
Histopathological, Immunological, Hematological and Biochemical Effects of Fipronil on Nile Tilapia (Oreochromis Niloticus) Abd elhakeem El-Murr.et al,Journal of Veterinary Science & Technology,2015
Dietary leucine requirement of Juvenile Nile tilapia, Oreochromis niloticus Sarker PK. et al,Fish Physiol Biochem,2012
Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita Lee JS et al,Fish Shellfish Immunol,2012
Effects of dietary supplementation of Lactobacillus pentosus PL11 on the growth performance, immune and antioxidant systems of Japanese eel Anguilla japonica challenged with Edwardsiella tarda Lee JS et al,Fish Shellfish Immunol,2012
Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita Giri SS et al,Fish Shellfish Immunol,2012
The use of kefir as potential probiotic in Çoruh trout (Salmo coruhensis): Effects on growth performance and immunoglobulin (IgM) levels Erkan Can et al,African Journal of Biotechnology,2012
Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides /,fish & shellfish immunology,2010
Dietary threonine requirement of juvenile Nile tilapia, Oreochromis niloticus Liebert F et al,J Anim Physiol Anim Nutr (Berl),2009

■ Q&A

CUSABIO fish immnoglobulin M (IgM) ELISA kit Does this kit can be used for separate experiments?around 30-40 wells every time?

Thanks for your inquiry.
#CSB-E12045Fh,Fish immunoglobulin M (IgM) ELISA Kit
YES,it can.The plate is Breakapart and can be used for seperate experiments.You can put the break part in the
Aluminum foil bag for next use.The manual is provided to you by e-mail.Additionally,we received 21 published papers until now.You can refer to it. Pls let me know if you have any further questions. Thank you.

Regarding your ELISA kit CSB-E12045Fh, could you please advise on the following:
1. Capture Antibody: host animal species, clonality
2. Detection Antibody: host animal species, clonality
3. Standard
Also, what sample types have been tested with this kit, and what were the reference values?

Thanks for your inquiry.
CSB-E12045Fh
The target is a special protein and there is no uniprot.
This kit is based on competitive method. The plate is coated with goat-anti-rabbit antibody. The specific antibody is rabbit polyclonal antibody. The standard is purified crude extract of natural samples.
We tested undiluted serum and plasma before and the test value is 1.25--3.75μg/ml.
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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