Fish thyroid stimulating hormone(TSH) ELISA Kit

Code CSB-EQ027261FI
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
thyroid stimulating hormone(TSH)
Alternative Names
tshb ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation
TSH
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
2.5 μIU/mL-40 μIU/mL
Sensitivity
2.5 μIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 100  
Range % 96-106  
1:2 Average % 89  
Range % 84-94  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 96  
Range % 91-101  
Recovery
The recovery of fish TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 86-93  
EDTA plasma (n=4) 95 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average    
40 0.234 0.228 0.231    
20 0.511 0.502 0.507    
10 0.888 0.893 0.891    
5 1.743 1.727 1.735    
2.5 2.557 2.458 2.508    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Fish TSH ELISA Kit was designed for the quantitative measurement of Fish TSH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 μIU/mL-40 μIU/mL and the sensitivity is 2.5 μIU/mL .

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Target Background

Function
(From Uniprot)
Indispensable for the control of thyroid structure and metabolism. May play some role in the biological processes of the immature fishes.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Tissue Specificity
Pituitary gland. Higher levels seen in immature fishes than the mature fishes.
Database Links

UniGene: Omy.8084

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