Code | CSB-E12914Gp |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 102 | ||||
Range % | 98-108 | |||||
1:2 | Average % | 94 | ||||
Range % | 91-98 | |||||
1:4 | Average % | 103 | ||||
Range % | 97-108 | |||||
1:8 | Average % | 88 | ||||
Range % | 80-96 |
The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 88-100 | ||||
EDTA plasma (n=4) | 104 | 96-108 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Guinea pig interleukin 2 (IL-2) ELISA Kit is a powerful tool for researchers in the field of immunology. The kit is designed for quantitative measurement of interleukin-2 protein in samples such as serum, plasma, and tissue homogenates of Cavia porcellus (Guinea pig).
The kit offers a broad detection range of 31.25 pg/mL-2000 pg/mL and a high sensitivity of 7.8 pg/mL, enabling accurate and reliable measurement of IL-2 levels in even low-concentration samples. With an assay time of 1-5 hours and a sample volume requirement of only 50-100ul, our kit is efficient and easy to use.
This Guinea pig IL-2 ELISA Kit employs a sandwich assay principle, where the target IL-2 protein is captured by a specific antibody and detected by a second antibody conjugated to a substrate. And the detection wavelength is 450 nm.
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