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Guinea pig interleukin 2,IL-2 ELISA Kit

Citations (1) Protocol MSDS
Code CSB-E12914Gp
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 2,IL-2
Abbreviation
IL-2
Uniprot No.
H0VBY4
Species
Cavia porcellus (Guinea pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %102
Range %98-108
1:2Average %94
Range %91-98
1:4Average %103
Range %97-108
1:8Average %88
Range %80-96
Recovery
The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-100
EDTA plasma (n=4)10496-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.230 2.351 2.291 2.152
10001.713 1.794 1.754 1.615
5001.214 1.235 1.225 1.086
2500.743 0.732 0.738 0.599
1250.495 0.464 0.480 0.341
62.50.349 0.338 0.344 0.205
31.250.255 0.251 0.253 0.114
00.139 0.138 0.139 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Guinea pig interleukin 2 (IL-2) ELISA Kit is a powerful tool for researchers in the field of immunology. The kit is designed for quantitative measurement of interleukin-2 protein in samples such as serum, plasma, and tissue homogenates of Cavia porcellus (Guinea pig).

The kit offers a broad detection range of 31.25 pg/mL-2000 pg/mL and a high sensitivity of 7.8 pg/mL, enabling accurate and reliable measurement of IL-2 levels in even low-concentration samples. With an assay time of 1-5 hours and a sample volume requirement of only 50-100ul, our kit is efficient and easy to use.

This Guinea pig IL-2 ELISA Kit employs a sandwich assay principle, where the target IL-2 protein is captured by a specific antibody and detected by a second antibody conjugated to a substrate. And the detection wavelength is 450 nm.

Citations

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