Horse Beta-Endorphin,β-EP ELISA Kit

Instructions
Code CSB-E06831Eq
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Beta-Endorphin,β-EP
Abbreviation β-EP
Species Equus caballus (Horse)
Sample Types serum, plasma
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 90-111
1:2 Average % 105
Range % 102-109
1:4 Average % 93
Range % 90-97
1:8 Average % 88
Range % 85-93
Recovery
The recovery of horse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 99-105
EDTA plasma (n=4) 91 90-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 1.899 1.922 1.911 1.788
100 1.554 1.514 1.534 1.411
50 1.123 1.078 1.101 0.978
25 0.615 0.623 0.619 0.496
12.5 0.421 0.456 0.439 0.316
6.25 0.277 0.266 0.272 0.149
3.12 0.185 0.194 0.190 0.067
0 0.126 0.120 0.123  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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