Human 14-3-3 protein beta/alpha (YWHAB) ELISA kit

Instructions
Code CSB-E12010h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
Alternative Names 14 3 3 ELISA Kit; 14 3 3 protein beta ELISA Kit; 14 3 3 protein beta/alpha ELISA Kit; 14 3 3 protein zeta ELISA Kit; 14 3 3 zeta ELISA Kit; 14-3-3 protein beta/alpha ELISA Kit; 14-3-3 protein/cytosolic phospholipase A2 ELISA Kit; 1433B_HUMAN ELISA Kit; GW128 ELISA Kit; HS1 ELISA Kit; KCIP 1 ELISA Kit; KCIP-1 ELISA Kit; MGC111427 ELISA Kit; MGC126532 ELISA Kit; MGC138156 ELISA Kit; N-terminally processed ELISA Kit; Protein 1054 ELISA Kit; Protein kinase C inhibitor protein 1 ELISA Kit; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; delta polypeptide ELISA Kit; Tyrosine 3/tryptophan 5 -monooxygenase activation protein; zeta polypeptide ELISA Kit; YWHAB ELISA Kit; YWHAD ELISA Kit; YWHAZ ELISA Kit
Abbreviation YWHAB
Uniprot No. P31946
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human YWHAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %94
Range %90-98
1:4Average %89
Range %84-93
1:8Average %84
Range %80-88
Recovery
The recovery of human YWHAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-95
EDTA plasma (n=4)9791-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
4002.552 2.602 2.577 2.456
2001.749 1.797 1.773 1.652
1001.028 1.083 1.056 0.935
500.569 0.543 0.556 0.435
250.338 0.316 0.327 0.206
12.50.246 0.240 0.243 0.122
6.250.167 0.161 0.164 0.043
00.125 0.116 0.121  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Gene References into Functions
  1. 14-3-3beta protein activates Pseudomonas exotoxin-S and exotoxin-T ADP-ribosyltransferase domains by chaperoning their hydrophobic surfaces independently of the amphipathic C-terminal segment. PMID: 30224724
  2. Data show that 14-3-3beta protein augmented the expression of matrix metalloproteinasea MMP2 and MMP9 through PI3 kinase/Akt protein/NF-kappaappa B pathway, thereby enhancing the invasiveness of hepatocellular carcinoma (HCC) cells. PMID: 26730736
  3. These findings indicate that 14-3-3beta and gamma are novel PPARgamma2 regulators and are involved in hepatic lipid metabolism. 14-3-3b and gamma can be therapeutic target molecules to treat non-alcoholic fatty liver disease. PMID: 26260846
  4. miR-152 controls both the expression of 14-3-3beta and HLA-G and exerts a dual role in tumor cells by both altering the immunogenicity and the tumorigenicity PMID: 25228695
  5. Data suggest that serum 14-3-3beta concentrations may constitute a useful marker for blood brain barrier damage severity and follow up in patients with eosinophilic meningitis caused by Angiostrongylus cantonensis. PMID: 24555778
  6. Using gene reporter assays, we show that promoter variations in 11 intrinsic apoptosis genes, including ADPRT, APAF1, BCL2, BAD, BID, MCL1, BIRC4, BCL2L1, ENDOG, YWHAB, and YWHAQ, influence promoter activity in an allele-specific manner. PMID: 24038028
  7. Crystal structure of Myo1c/14-3-3beta complex, which has been implicated in the exocytosis of glucose transporter 4 storage vesicles during insulin-stimulated glucose uptake. PMID: 24636949
  8. Data identified three classes of 14-3-3 targets that all have two binding sites, but displayed synergistic interaction between converging signalling pathways for different ranges of parameter values. PMID: 24269229
  9. These results indicate that the six YWHAB polymorphisms are not associated with the genetic susceptibility to sporadic Creutzfeldt-Jakob disease. PMID: 23053962
  10. 14-3-3beta binding to phosphorylated CFTR augments its biogenesis by reducing retrograde retrieval of CFTR to the endoplasmic reticulum. This mechanism permits cAMP/PKA stimulation to make more CFTR available for anion secretion. PMID: 22278744
  11. Data identified 14-3-3beta as a prognostic biomarker. PMID: 22125622
  12. Modulation of matrix metalloproteinase 1 by 14-3beta/alpha, may be important in the alteration of collagenase production associated with airway remodelling in obstructive lung diseases PMID: 21948273
  13. Analyses show that high cytoplasmic levels of 14-3-3beta and epsilon independently correlate with poor disease-specific survival in vulvar squamous cell carcinoma cases. PMID: 21935479
  14. In glioblastoma PTPIP51 expression increases with the grade of malignancy and PTPIP51 interacts in situ with 14-3-3ss and PTP1B. PMID: 21972092
  15. Studies indicate that Akt phosphorylates acetylated-FoxO and then phosphorylated FoxO interacts with 14-3-3 proteins in the nucleus, which in turn results in cytoplasmic retention of FoxO. PMID: 21396404
  16. Studies indictet that the mammalian FoxO family consists of FoxO1, 3, 4 and 6 and are regulated by by AKT and 14-3-3 proteins. PMID: 21708191
  17. The expression levels of 14-3-3 protein beta/alpha were higher in urine samples from patients with renal cell carcinoma than in samples from healthy volunteers. PMID: 21553213
  18. Data indicate that gene analysis revealed an up-regulation of all four 14-3-3 isoforms beta, eta, gamma, and sigma. PMID: 21416292
  19. 14-3-3beta protein has the potential to be used as a diagnostic and prognostic biomarker in gastric cancer. PMID: 21598387
  20. 14-3-3beta interacts with human Dapper1, attenuating the ability of hDpr1 to promote Dishevelled (Dvl) degradation, thus enhancing Wnt signaling PMID: 21262972
  21. protein within PIV5-infected cells is phosphorylated at residue S369, binds the 14-3-3 protein, and is held away from sites of virus budding. PMID: 21147917
  22. 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma PMID: 20388496
  23. Show that viral infection activates 14-3-3 protein mediated signaling pathways in human keratinocytes. PMID: 20070120
  24. Study identified an overrepresentation of focal amplifications of known (FGFR3, CCND1, MYC, MDM2) and novel candidate genes (MYBL2, YWHAB and SDC4) in stage Ta bladder carcinoma. PMID: 19821490
  25. 14-3-3beta, 14-3-3gamma, 14-3-3epsilon, 14-3-3eta and 14-3-3theta isoforms interact with the GPIb-IX complex in platelets PMID: 19558434
  26. Results show that three 14-3-3 isoforms, beta, gamma and eta, are DAL-1/Protein 4.1B-binding proteins. PMID: 11996670
  27. TSC2 associates with 14-3-3 in vivo PMID: 12364343
  28. KCNK3 potassium channels are shown to bear two cytoplasmic trafficking motifs: an N-terminal dibasic site that binds beta-COP to hold channels in ER and a C-terminal "release" site that binds the ubiquitous intracellular regulator 14-3-3beta PMID: 12437930
  29. 14-3-3 beta interacts with the TSC1-TSC2 complex and negatively regulates the function of the TSC proteins PMID: 12468542
  30. 14-3-3 binds to the IGF-1 receptor after IGF1R's serine autophosphorylation PMID: 12482592
  31. MK2 phosphorylates TSC2, which creates a 14-3-3 binding site and thus regulates the cellular function of the TSC2 tumor suppressor protein PMID: 12582162
  32. 14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity PMID: 12618428
  33. Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy. PMID: 12669242
  34. These findings suggest that deregulation of 14-3-3 protein amounts might contribute to the development of tumors in tuberous sclerosis patients. PMID: 14680818
  35. HS1 with EPEP insertion polymorphism transmits accelerated signals from B cell receptor and is involved in pathogenesis of systemic lupus erythematosus. PMID: 15022330
  36. novel binding site on 14-3-3 for integrin beta1 and a functional amphipathic groove, rather than its interaction with integrin beta1, is required for 14-3-3 regulation of cell spreading and migration. PMID: 15389601
  37. decreased expression of selected 14-3-3 genes is a common feature of schizophrenia PMID: 15726117
  38. These data show a novel interaction for 14-3-3 with NFL mRNA, and suggests that 14-3-3 may play a role in regulating NFL mRNA stability. PMID: 17098443
  39. 14-3-3beta binds DYRK1A PMID: 17229891
  40. PBF is a new cellular factor mediating the effects of PI3K/Akt signaling and 14-3-3 on cell growth PMID: 17531190
  41. Ror2 induces osteogenic differentiation, at least in part, through a release of the 14-3-3beta-mediated inhibition PMID: 17717073
  42. A new regulatory mechanism of myosin light-chain phosphatase via the interaction between 14-3-3 and MYPT1, is reported. PMID: 18094049
  43. changes in the expression of five 14-3-3 isoforms (beta, gamma, epsilon, tau, and zeta) during the apoptosis of JURL-MK1 and K562 cells. PMID: 19173300
  44. Tyrosine 3-monooxygenase/tryptophan S-monooxygenase activation protein, beta polypeptide is decreased during acute lung injury more in mice deficient in metallothionein 1/2 PMID: 16166738

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Subcellular Location Cytoplasm, Melanosome
Protein Families 14-3-3 family
Database Links

HGNC: 12849

OMIM: 601289

KEGG: hsa:7529

STRING: 9606.ENSP00000300161

UniGene: Hs.643544

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