Code | CSB-RA616077A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The creation of the YWHAB recombinant monoclonal antibody is a complicated process with several steps involved. Initially, the YWHAB monoclonal antibody is obtained, and its gene sequence is determined. Then, a vector carrying the YWHAB monoclonal antibody gene is constructed and transfected into a host cell line for culture. The YWHAB monoclonal antibody is produced using a synthesized peptide derived from human YWHAB as an immunogen. Afterward, the resulting YWHAB monoclonal antibody is purified through affinity chromatography from cell culture supernatant and analyzed for specificity using ELISA, WB, IF, and FC applications. It can react with three species, including human, mouse, and rat.
The YWHAB protein, also known as 14-3-3 beta protein, belongs to the 14-3-3 family of proteins that are involved in the regulation of multiple cellular processes such as signal transduction, cell cycle control, apoptosis, and protein trafficking. YWHAB interacts with a wide range of proteins, including transcription factors, enzymes, and structural proteins, and regulates their activity and localization within the cell. It is involved in the regulation of various signaling pathways, such as the AKT and MAPK pathways, and is important for cell proliferation, differentiation, and survival. Furthermore, YWHAB has been implicated in the pathogenesis of several diseases, including cancer, neurodegenerative disorders, and metabolic diseases, indicating its crucial role in maintaining cellular homeostasis.
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